NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE104810 Query DataSets for GSE104810
Status Public on Oct 10, 2018
Title Genome-wide mapping of H3K79me2 in EPZ5676-treated lymphatic endothelial cells [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Proper functioning of the lymphatic system is required for normal immune response, fluid balance and lipid reabsorption. Multiple regulatory mechanisms are employed to ensure correct formation of lymphatic vessels; however, whether epigenetic regulation is involved in this process remains largely unknown. We report that epigenetic priming by the Disruptor of telomeric silencing 1-like (DOT1L) in endothelial cell (EC) is indispensable for generation and function of lymphatic endothelial cells (LECs). Mechanistically, loss function of DOT1L leads to reduction of H3K79 di-methylation and expression of the genes important for LEC development and function. Thus, our study establishes DOT1L-mediated transcriptional regulation in ECs plays an important role in differentiation and function of LECs.
 
Overall design We perform RNA-Seq experiment in mouse E15.5 skin LECs (control vs Dot1l KO).
 
Contributor(s) Yoo H, Park CH, Zhang Y, Choi Y, Hong K
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 11, 2017
Last update date Jul 25, 2021
Contact name Chanhyeok Park
E-mail(s) chpark0729@gmail.com
Organization name Konkuk University
Department Stem cell and regeneration biotechnology
Lab Hong lab
Street address 120, Neungdong-ro
City Seoul
ZIP/Postal code 05066
Country South Korea
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (2)
GSM2808237 Cont_LEC
GSM2808238 MT_LEC
This SubSeries is part of SuperSeries:
GSE104811 Genome-wide mapping of H3K79me2 in EPZ5676-treated lymphatic endothelial cells
Relations
BioProject PRJNA413925
SRA SRP119747

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104810_RAW.tar 280.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap