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Series GSE104555 Query DataSets for GSE104555
Status Public on Oct 04, 2017
Title Chemical reprogramming of mouse embryonic and adult fibroblast into endoderm lineage
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report here an approach to redirect somatic cell fate under chemically defined conditions without transcription factors. We start by converting mouse embryonic fibroblasts (MEFs) to epithelial-like cells (ciELC) with chemicals and growth factors. Subsequent cell fate mapping reveals a robust induction of SOX17 in the resulting ELCs that can be further reprogrammed to endodermal progenitor cells (ciEPC). Interestingly, these cells can self-renew in vitro and further differentiate into albumin-producing hepatocytes that can rescue mice from acute live injury. Our results demonstrate a rational approach to convert MEFs to hepatocytes and suggest that this mechanism-driven approach may be generalized for other cells.
 
Overall design Examination of mouse fibroblasts, ciEPCs, ciHeps, ESC derived EPCs and primary hepatocytes
 
Contributor(s) Liu J, Pei D
Citation(s) 28935668
Submission date Oct 03, 2017
Last update date Jul 25, 2021
Contact name Lihui Lin
E-mail(s) lin_lihui@gibh.ac.cn
Phone 020-32015291
Organization name Guangzhou Institutes Of Biomedicine and Health Chinese Academy Of Science
Department Stem cell
Lab Pei Duanqing
Street address No.190, Kaiyuan Street
City Guangzhou
State/province Guangdong
ZIP/Postal code GD20
Country China
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (28)
GSM2803306 MEF1
GSM2803307 MEF2
GSM2803308 MEF3
Relations
BioProject PRJNA413045
SRA SRP119326

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104555_RAW.tar 21.8 Mb (http)(custom) TAR (of TXT)
GSE104555_raw_counts.csv.gz 1.4 Mb (ftp)(http) CSV
GSE104555_tpm.csv.gz 1.5 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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