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Status |
Public on Oct 19, 2017 |
Title |
Poly(A) Tail Length of L4 C. elegans |
Organism |
Caenorhabditis elegans |
Experiment type |
Expression profiling by high throughput sequencing Other
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Summary |
Poly(A) tails are important elements in mRNA translation and stability. However, recent genome-wide studies concluded that poly(A) tail length was generally not associated with translational efficiency in non-embryonic cells. To investigate if poly(A) tail size might be coupled to gene expression in an intact organism, we used an adapted TAIL-seq protocol to measure poly(A) tails in Caenorhabditis elegans. Surprisingly, we found that well-expressed transcripts contain relatively short, well-defined tails. This attribute appears dependent on translational efficiency, as transcripts enriched for optimal codons and ribosome association had the shortest tail sizes, while non-coding RNAs retained long tails.
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Overall design |
Global investigation of L4 C. elegans poly(A) tail length by using mTAIL-seq and analysis by the tailseeker2 program. Two independent biological replicates were used.
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Contributor(s) |
Azoubel Lima S, Chipman LB, Nicholson AL |
Citation(s) |
29106412 |
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Submission date |
Oct 02, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Amy Pasquinelli |
E-mail(s) |
apasquinelli@ucsd.edu
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Organization name |
University of California, San Diego
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Department |
Biological Sciences
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Lab |
Pasquinelli Lab
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Street address |
9500 Gilman Drive
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City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
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Platforms (1) |
GPL15716 |
Illumina MiSeq (Caenorhabditis elegans) |
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Samples (2) |
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This SubSeries is part of SuperSeries: |
GSE104502 |
Short Poly(A) Tails are a Conserved Feature of Highly Expressed Genes |
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Relations |
BioProject |
PRJNA412860 |
SRA |
SRP119251 |