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Series GSE104501 Query DataSets for GSE104501
Status Public on Oct 19, 2017
Title Poly(A) Tail Length of L4 C. elegans
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Other
Summary Poly(A) tails are important elements in mRNA translation and stability. However, recent genome-wide studies concluded that poly(A) tail length was generally not associated with translational efficiency in non-embryonic cells. To investigate if poly(A) tail size might be coupled to gene expression in an intact organism, we used an adapted TAIL-seq protocol to measure poly(A) tails in Caenorhabditis elegans. Surprisingly, we found that well-expressed transcripts contain relatively short, well-defined tails. This attribute appears dependent on translational efficiency, as transcripts enriched for optimal codons and ribosome association had the shortest tail sizes, while non-coding RNAs retained long tails.
 
Overall design Global investigation of L4 C. elegans poly(A) tail length by using mTAIL-seq and analysis by the tailseeker2 program. Two independent biological replicates were used.
 
Contributor(s) Azoubel Lima S, Chipman LB, Nicholson AL
Citation(s) 29106412
Submission date Oct 02, 2017
Last update date May 15, 2019
Contact name Amy Pasquinelli
E-mail(s) apasquinelli@ucsd.edu
Organization name University of California, San Diego
Department Biological Sciences
Lab Pasquinelli Lab
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL15716 Illumina MiSeq (Caenorhabditis elegans)
Samples (2)
GSM2802532 WT rep1 (mTAIL-seq)
GSM2802533 WT rep2 (mTAIL-seq)
This SubSeries is part of SuperSeries:
GSE104502 Short Poly(A) Tails are a Conserved Feature of Highly Expressed Genes
Relations
BioProject PRJNA412860
SRA SRP119251

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104501_N2_TailLength.csv.gz 21.0 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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