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| Status |
Public on Dec 05, 2017 |
| Title |
Transcriptional Responses in Mouse Models of Invasive Pulmonary Aspergillosis |
| Organisms |
Mus musculus; Aspergillus fumigatus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Abstract From manuscript: Incidences of invasive pulmonary aspergillosis, an infection caused predominantly by Aspergillus fumigatus, have increased due to the growing number of immunocompromised individuals. While A. fumigatus is reliant upon deficiencies in the host to facilitate invasive disease, the distinct mechanisms that govern the host-pathogen interaction remain enigmatic, particularly in the context of distinct immune modulating therapies. To gain insights into these mechanisms, RNA-Seq technology was utilized to sequence RNA derived from lungs of 2 clinically relevant, but immunologically distinct murine models of IPA on days 2 and 3 post inoculation when infection is established and active disease present. Our findings identify notable differences in host gene expression between the chemotherapeutic and steroid models at the interface of immunity and metabolism. RT-qPCR verified model specific and nonspecific expression of 23 immune-associated genes. Deep sequencing facilitated identification of highly expressed fungal genes. We utilized sequence similarity and gene expression to categorize the A. fumigatus putative in vivo secretome. RT-qPCR suggests model specific gene expression for nine putative fungal secreted proteins. Our analysis identifies contrasting responses by the host and fungus from day 2 to 3 between the two models. These differences may help tailor the identification, development, and deployment of host- and/or fungal-targeted therapeutics.
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| Overall design |
Three biological replicates were conducted for each model (chemotherapeutic and steroid) and time point (day 2 and day 3 post inncoulation) resulting in a total of 12 samples. One replicate for the chemotherapeutic model for day 2 failed during sequencing analysis, and therefore that model and time point only has 2 biological replicates. The successful 11 samples data are presented.
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| Contributor(s) |
Kale SD, Ayubi T, Chung D, Tubau-Juni N, Leber A, Dang HX, Karyala S, Hontecillas R, Lawrence CB, Cramer RA, Bassaganya-Riera J |
| Citation(s) |
29213115 |
| |
| Submission date |
Sep 26, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Shiv D. Kale |
| E-mail(s) |
sdkale@vt.edu
|
| Phone |
540-231-3784
|
| Organization name |
Virginia Tech
|
| Department |
Biocomplexity Institute
|
| Street address |
1015 Steger Hall, Life Science Circle
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24049 |
Illumina HiSeq 2000 (Aspergillus fumigatus; Mus musculus) |
|
| Samples (11)
|
| GSM2794441 |
LD2_2 Chemotherapeutic Model Day 2 Post Infection |
| GSM2794442 |
LD2_4 Chemotherapeutic Model Day 2 Post Infection |
| GSM2794443 |
LD3_1 Chemotherapeutic Model Day 3 Post Infection |
| GSM2794444 |
LD3_3 Chemotherapeutic Model Day 3 Post Infection |
| GSM2794445 |
LD3_5 Chemotherapeutic Model Day 3 Post Infection |
| GSM2794446 |
SD2_1 Steroid Model Day 2 Post Infection |
| GSM2794447 |
SD2_2 Steroid Model Day 2 Post Infection |
| GSM2794448 |
SD2_5 Steroid Model Day 2 Post Infection |
| GSM2794449 |
SD3_1 Steroid Model Day 3 Post Infection |
| GSM2794450 |
SD3_2 Steroid Model Day 3 Post Infection |
| GSM2794451 |
SD3_3 Steroid Model Day 3 Post Infection |
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| Relations |
| BioProject |
PRJNA412263 |
| SRA |
SRP118939 |
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