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Series GSE104219 Query DataSets for GSE104219
Status Public on Jan 31, 2021
Title Gene expression program directed by an artificially evolved POU (ePOU)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We generated Oct4 libraries by randomizing selected amino acids and by recombining domains of paralogous POU family genes. These libraries were subjected to iterative rounds of pooled screens to select variants that enhance pluripotency induction. We identified an artificially evolved POU factor (ePOU) that substantially outperforms wild-type Oct4 in terms of mouse embryonic fibrobast (MEF) reprogramming. We compared the transcriptomes of cells reprogramming under ePOU or Oct4 conditions at day 3 using RNA sequencing.
 
Overall design mRNA profiles of 24 different reprogramming intermediate cells with ePOU/Oct4 in combination with Sox2/Sox17EK along with Klf4 and c-Myc, at day 0,2,3,4,6,and 13 of retrovirus system based
 
Contributor(s) Chen Y, Jauch R, Malik V, Tan D
Citation(s) 33720298
Submission date Sep 25, 2017
Last update date May 02, 2021
Contact name Chen yanpu
E-mail(s) dsytan@connect.hku.hk
Phone +85255395054
Organization name Guangzhou Institute of Biomedicine and Health
Department drug discovery pipelines
Lab Ralf
Street address Kaiyuan No. 190, Luogang district
City Guangzhou
State/province guangdong
ZIP/Postal code 510000
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (45)
GSM2792773 RNAseq_day3_reprogramming_OSKM_rep1
GSM2792774 RNAseq_day3_reprogramming_OSKM_rep2
GSM2792775 RNAseq_day3_reprogramming_ePOUSKM_rep1
Relations
BioProject PRJNA412048
SRA SRP118812

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104219_ePOU_Oct4_genes_ntc_filtered_expression.tsv.gz 1.1 Mb (ftp)(http) TSV
GSE104219_genes_ntc_expression.txt.gz 390.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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