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| Status |
Public on Sep 21, 2020 |
| Title |
Validation of AS1 as novel regulator of tumor resistance to immune cell attack |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The experiment was performed to verify wether knockdown of AS1 in combination with TNFa treatment affected the signature of genes involved in tumor cell apoptosis and survival
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| |
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| Overall design |
The experiment was conducted with biological replicates. PANC-1 WT cells were reverse transfected either with siCtr1 or with siAS1 s1 (siRNA sequences) in a 6-well plate . Afterwards, the medium was replaced with 1 mL of complete DMEM and 1 mL rHuTNF-α was added at a final concentration of 100 ng/mL. After 30 min or 4h from stimulation, tumor cells were harvested in trypsin and RNA was isolated using the RNeasy mini kit (Qiagen) according to the manufacturer´s instructions.
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| |
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| Contributor(s) |
Sorrentino A, Rehli M, Gebhard C |
| Citation(s) |
35606086 |
| Submission date |
Sep 21, 2017 |
| Last update date |
Jun 27, 2022 |
| Contact name |
Claudia GEBHARD |
| E-mail(s) |
claudia.gebhard@gmail.com
|
| Organization name |
UNIVERSITY HOSPITAL REGENSBURG
|
| Street address |
FRANZ-JOSEF-STRAUSS ALLEE 11
|
| City |
REGENSBURG |
| ZIP/Postal code |
93042 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE202305 |
RNA-seq and ATAC-seq of tumor resistance to immune cell attack |
|
| Relations |
| BioProject |
PRJNA408285 |
| SRA |
SRP118511 |
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