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Series GSE104112 Query DataSets for GSE104112
Status Public on Sep 21, 2020
Title Validation of AS1 as novel regulator of tumor resistance to immune cell attack
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The experiment was performed to verify wether knockdown of AS1 in combination with TNFa treatment affected the signature of genes involved in tumor cell apoptosis and survival
 
Overall design The experiment was conducted with biological replicates. PANC-1 WT cells were reverse transfected either with siCtr1 or with siAS1 s1 (siRNA sequences) in a 6-well plate . Afterwards, the medium was replaced with 1 mL of complete DMEM and 1 mL rHuTNF-╬▒ was added at a final concentration of 100 ng/mL. After 30 min or 4h from stimulation, tumor cells were harvested in trypsin and RNA was isolated using the RNeasy mini kit (Qiagen) according to the manufacturer┬┤s instructions.
 
Contributor(s) Sorrentino A, Rehli M, Gebhard C
Citation(s) 35606086
Submission date Sep 21, 2017
Last update date Jun 27, 2022
Contact name Claudia GEBHARD
E-mail(s) claudia.gebhard@gmail.com
Organization name UNIVERSITY HOSPITAL REGENSBURG
Street address FRANZ-JOSEF-STRAUSS ALLEE 11
City REGENSBURG
ZIP/Postal code 93042
Country Germany
 
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (12)
GSM2790124 siCtrl1 Unst. Rep1
GSM2790125 siCtrl1 Unst. Rep2
GSM2790126 siAS1 Unst. Rep1
This SubSeries is part of SuperSeries:
GSE202305 RNA-seq and ATAC-seq of tumor resistance to immune cell attack
Relations
BioProject PRJNA408285
SRA SRP118511

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104112_RAW.tar 3.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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