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Series GSE104111 Query DataSets for GSE104111
Status Public on Jan 15, 2018
Title BCR heavy and light chain repertoire sequencing of pertitoneal cavity B-1a cells from wild-type and Igll1 knockout mice
Organism Mus musculus
Experiment type Other
Summary We report that B-1a cells develop in a surrogate light chain independent context. As a consequence, the precursor B-1a cell population avoids a pre-BCR positive selection stage. To confirm that the B-1a cells generated in this manner repersent a bonafide B-1a cell compartment, we did NGS on BCR rearrangements to assess the repertoire diversity. We find that as a whoile, B-1a cell repertoire that develop in Igll1 kncokout mice are similar compared to wild-type. This supports our findings that B-1a cells develop properly in the absence of surrogate light chain.
Overall design Peritoneal cavity B-1a cells were sorted from 6-8 week old mice, DNA was extracted and rearranged heavy chains were amplified by PCR. These PCR products were then process to form DNA libraries that were compatible with Illumina NGS.
Contributor(s) Skok JA, Wong JB
Citation(s) 31628339
Submission date Sep 21, 2017
Last update date Jul 25, 2021
Contact name Jane Skok
Organization name NYU
Street address 550 First Ave, Smillow 307
City New York
State/province NY
ZIP/Postal code 10016
Country USA
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (10)
GSM2790120 WT PerC B-1a Rep1
GSM2790121 WT PerC B-1a Rep2
GSM2790122 Igll1 PerC B-1a Rep1
BioProject PRJNA408276
SRA SRP118496

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104111_RAW.tar 11.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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