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Series GSE104110 Query DataSets for GSE104110
Status Public on Sep 21, 2018
Title Transcriptional profile of human neonatal cord blood and adult peripheral blood dendritic cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A phenotypically and functinoally distinct subset of human blood dendritic cells expressing CD11b is specific of the neonatal environment. We have employed whole genome microarray expression profiling to identify the specific gene signature of CD11b+ cord blood dendritic cells as compared to their adult peripheral blood counterparts. Peripheral blood adult cDC2 (CD20- CD11c+ CD14- BDCA1+ CD11b- ), neonatal cord blood cDC2 (CD20- CD11c+ CD14- BDCA1+ CD11b-) and neonatal cord blood cDC2b (CD20- CD11c+ CD14- BDCA1+ CD11b+) were FACS purified from BDCA1+ magnetically. Neonatal monocytes (CD11c+ CD14+) and neonatal naive T cells (CD3+ CD4+ CD56- CD25- CD45RO-) were used as controls.
Overall design Dendritic cell subsets were isolated from 3 peripheral blood donors and 3 cord blood donors. Monocytes were isolated from 3 cord blood donors. Neonatal naive T cells were isolated from 1 peripheral blood donor. Dendritic cells were left unstimulated or were stimulated with either poly I:C + R848, Bacillus Calmette–Guérin (BCG) or Respiratory Syncytial Virus (RSV) for 6h. RNA from 5000 cells was amplified, labeled, and hybridized to an an Agilent Whole Human Genome Oligo Microarrays 8x60K.
Contributor(s) Lemoine S, Zhang X, Lo-Man R
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Submission date Sep 21, 2017
Last update date Jul 25, 2021
Contact name Sebastien LEMOINE
Organization name Necker Hospial
Lab INSERM U1151 CNRS UMR 8253
Street address 149 rue de Sèvres, Bat. Hamburger, 5e étage, p507
City Paris
ZIP/Postal code 75016
Country France
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (40)
GSM2790080 adult_cDC2_none_1
GSM2790081 adult_cDC2_TLR_1
GSM2790082 adult_cDC2_BCG_1
BioProject PRJNA408282

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104110_RAW.tar 499.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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