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Series GSE104084 Query DataSets for GSE104084
Status Public on Sep 20, 2017
Title Measuring RNA editing through mmPCR-seq in Drosophila adapting to divergent microclimates and raised at different temperatures
Organism Drosophila melanogaster
Experiment type Other
Summary We applied microfluidic multiplex PCR and deep sequencing (mmPCR-seq) to quantify RNA editing levels at targeted sites in 32 isofemale lines from two divergent microclimates at 'Evolution Canyon' in Israel (16 fly lines from each microclimate). Editing levels were compared between different populations at 25˚C , and were also compared between 25˚C and 18˚C within populations.
Overall design For each of the 32 isofemale fly lines from 'Evolution Canyon', quantification of RNA editing levels in the whole bodies of 3-5 day old male flies raised at 25˚C or 18˚C through RT-PCR amplification of 605 loci. Two biological replicates are represented per fly line.
Contributor(s) Yablonovitch AL, Li K, Rashkovetsky E, Korol AB, Michalak P, Nevo E, Li JB
Citation(s) 29146998
Submission date Sep 20, 2017
Last update date Jul 25, 2021
Contact name Jin Billy Li
Organization name Stanford University
Department Genetics
Street address 300 Pasteur Drive, Alway M-341
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (2)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
Samples (128)
GSM2789303 N1-1_25C_mmPCR
GSM2789304 N1-2_25C_mmPCR
GSM2789305 N2-1_25C_mmPCR
This SubSeries is part of SuperSeries:
GSE104085 Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates
BioProject PRJNA408169
SRA SRP118387

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104084_RAW.tar 2.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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