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Series GSE103718 Query DataSets for GSE103718
Status Public on Sep 11, 2019
Title Active fluctuations of the nuclear envelope shape the transcriptional dynamics in oocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Nucleus position in cells can act as a developmental cue. Mammalian oocytes position their nucleus centrally using an F-actin mediated pressure gradient. The biological significance of nucleus centring in mammalian oocytes being unknown, we sought to assess the F-actin pressure gradient effect on the nucleus. We addressed this using a dedicated computational 3D imaging approach, biophysical analyses, and a nucleus repositioning assay in mouse oocytes mutant for cytoplasmic F-actin. We found that the cytoplasmic activity, in charge of nucleus-centring, shaped the nucleus while promoting nuclear envelope fluctuations and chromatin motion. Off-centred nuclei in F-actin mutant oocytes were misshaped with immobile chromatin and modulated gene expression. Restoration of F-actin in mutant oocytes rescued nucleus architecture fully and gene expression partially. Thus, the F-actin mediated pressure gradient also modulates nucleus dynamics in oocytes. Moreover, this study supports a mechano-transduction model whereby cytoplasmic microfilaments could modulate oocyte transcriptome, essential for subsequent embryo development.
Overall design 2 samples of Fmn2 +/- oocytes, 50 oocytes each and 2 samples of Fmn 2-/- oocytes, 50 oocytes each. For each biological sample, 3 technical replicates were done.
Contributor(s) Almonacid M, Al Jord A, El-Hayek S, Othmani A, Coulpier F, Lemoine S, Miyamoto K, Grosse R, Klein C, Piolot T, Mailly P, Voituriez R, Genovesio A, Verlhac M
Citation(s) 31607652, 36038550
Submission date Sep 11, 2017
Last update date Sep 15, 2022
Contact name Stephane Le Crom
Organization name IBENS
Department Genomic Core Facility
Street address 46 rue Ulm
ZIP/Postal code 75230
Country France
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (12)
GSM2779641 Fmn 7 a
GSM2779642 Fmn 7 b
GSM2779643 Fmn 7 c
BioProject PRJNA406820
SRA SRP117280

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Supplementary file Size Download File type/resource
GSE103718_RAW.tar 2.2 Mb (http)(custom) TAR (of TAB)
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Raw data are available in SRA
Processed data provided as supplementary file

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