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Series GSE103694 Query DataSets for GSE103694
Status Public on Nov 16, 2017
Title Baf53a is involved in survival of mouse ES cells, which can be compensated by Baf53b.
Organism Mus musculus
Experiment type Expression profiling by array
Summary The human Baf (Brg1/Brm associated factor) complex, also known as the mammalian SWI/SNF chromatin-remodeling complex, is involved in a variety of cellular processes. The pluripotency and self-renewal abilities are major characteristics of embryonic stem (ES) cells and are regulated by the ES cell-specific BAF (esBAF) complex. Baf53a is one of the subunits of the esBAF complex. Here, we found that growth of Baf53a-deficient ES cells was repressed, and expression of p53, p21, and cleaved Caspase 3 was increased. Interestingly, Baf53b, a homologue of Baf53a, rescued cell death of Baf53a-deficient ES cells. Baf53a-deficient ES cells overexpressing exogenous Baf53a or Baf53b remained in the undifferentiated state and proliferated. In summary, our findings suggest that Baf53a is involved in the survival of ES cells, and that Baf53b is able to compensate for this functional aspect of Baf53a.
Overall design Comaprison between Baf53a-expressing or Baf53b-expressing Baf53a-deficient ES cells.
Contributor(s) Zhu B, Ueda A, Song X, Horike S, Yokota T, Akagi T
Citation(s) 29070872
Submission date Sep 11, 2017
Last update date Jul 25, 2021
Contact name Shin-ichi Horike
Phone +81-76-265-2775
Organization name Kanazawa University
Department Research Center for Experimental Modeling of Human Disease
Street address 13-1 Takaramachi
City Kanazawa
State/province Ishikawa
ZIP/Postal code 920-0934
Country Japan
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (2)
GSM2779365 Baf53a-cKO cells+Baf53a
GSM2779366 Baf53a-cKO cells+Baf53b
BioProject PRJNA404073

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103694_RAW.tar 4.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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