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Series GSE103581 Query DataSets for GSE103581
Status Public on Sep 07, 2017
Title First trimester human placenta prevents breast cancer cell attachment to the matrix: the role of extracellular matrix
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The placenta is a nonsupportive microenvironment for cancer cells. We showed that breast cancer cells (BCCL) were eliminated from placental implantation sites. During implantation, the placenta manipulates its surrounding matrix, which may induce BCCL elimination. Here, we explored the effect of placenta-induced ECM manipulations on BCCL. During experiments, BCCL (MCF-7/T47D) were cultured on placenta/BCCL-conditioned ECM (Matrigel used for first trimester placenta/BCCL culture and cleared by NH4OH). After culturing the cells, we analyzed cancer cell phenotype (death, count, aggregation, MMP) and signaling (microarray analysis and pathway validation). We found that the BCCL did not attach to previous placental implantation sites and instead, similarly to anoikis-resistant cells, migrated away, displayed increased MMP levels/activity, and formed aggregates in distant areas. T47D were less affected than the MCF-7 cells, since MCF-7 also showed modest increases in cell death, EMT, and increased proliferation. Microarray analysis of the MCF-7 highlighted changes in the integrin, estrogen, EGFR, and TGFb pathways. Indeed, placental ECM reduced ERa, induced Smad3/JNK phosphorylation and increased integrin-a5 expression (RGD-dependent integrin) in the BCCL. Addition of RGD or TGFbR/JNK inhibitors reversed the phenotypic changes. This study helps explain the absence of metastases to the placenta and why advanced cancer is found in pregnancy, and provides possible therapeutic targets for anoikis-resistant cells.
 
Overall design Cells were cultured with or without placetal-conditioned extracellular matrix, and microarrays were used to estimate changes in gene expression.
Web link http://onlinelibrary.wiley.com/doi/10.1002/mc.22473/abstract
 
Contributor(s) Epstein Shochet G, Drucker L, Pomeranz M, Fishman A, Pasmanik-Chor M, Tartakover-Matalon S, Lishner M
Citation(s) 26859229
Submission date Sep 07, 2017
Last update date Jul 25, 2021
Contact name Metsada Pasmanik-Chor
E-mail(s) metsada@tauex.tau.ac.il
Organization name Tel Aviv University
Department Biology
Lab Bioinformatics Unit
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 69978
Country Israel
 
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (4)
GSM2774786 ECM rep1
GSM2774787 placenta-conditioned ECM rep1
GSM2774788 ECM rep2
Relations
BioProject PRJNA401987

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103581_RAW.tar 18.2 Mb (http)(custom) TAR (of CEL)
GSE103581_rma_gene_matrix.txt.gz 651.8 Kb (ftp)(http) TXT
Processed data included within Sample table

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