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Series GSE103353 Query DataSets for GSE103353
Status Public on Aug 25, 2020
Title The mRNA alteration in HT22 cells induced by the overexpression of long noncoding RNA AK046375
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We overexpressed the lncRNA-AK046375 by lentivirus in HT22 cells, which were immortalized from the mice hippocampal neurons. Then we measured the mRNA alteration induced by AK046375 overexpression through the next generation sequencing technique (RNA-seq). A total of 20,319 transcripts were detected in the current sequencing study, in which 1342 transcripts were selected out according to the criteria: |log2(fold change)| > 1, P < 0.05 (717 up-regulated and 625 down-regulated). To explore whether the mRNA change induced by AK046375 overexpression in a stressed culture condition was different from that in normal condition, the cells were dealt with oxygen-glucose-deprived(OGD) and followed by RNA-seq. A total of 22,016 transcripts were detected, in which 87 transcripts were significantly up-regulated and 131 transcripts were significantly down-regulated according to the aforementioned filter rules.
 
Overall design Firstly, HT22 cells were infected with lentivirus to overexpress AK046375. Secondly, cells were cultured in either normal or OGD condition. Finally, cells were collected to extract total RNA and subjected to next generation RNA sequencing analysis.
 
Contributor(s) Zhong J, Sun X
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 31, 2017
Last update date Jul 25, 2021
Contact name Zhong Jianjun
E-mail(s) zjjcom682161@126.com
Phone +86 18696786170
Organization name The first Affiliated Hospital of Chongqing Medical University
Department Neurosurgery Department
Street address NO.1 Youyi Road, Yuzhong District, Chongqing
City Chongqing
ZIP/Postal code 400016
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM2769063 1: control in normal condition
GSM2769064 2: AK046375 knockdown in normal condition
GSM2769065 3: control in OGD condition
Relations
BioProject PRJNA401694
SRA SRP131330

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103353_1_vs_2.xls.gz 219.0 Kb (ftp)(http) XLS
GSE103353_3_vs_4.xls.gz 41.5 Kb (ftp)(http) XLS
GSE103353_RAW.tar 7.2 Mb (http)(custom) TAR (of XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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