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Series GSE102389 Query DataSets for GSE102389
Status Public on Nov 01, 2017
Title Effect of group III phospholipase A2 deletion in azoxymethane-induced colon carcinogenesis modeled colon
Organism Mus musculus
Experiment type Expression profiling by array
Summary Lipid mediators play pivotal roles in colorectal cancer and colitis, but only a limited member of the phospholipase A2 (PLA2) subtypes, which lie upstream of various lipid mediators, have been implicated in the positive or negative regulation of these diseases. Clinical and biochemical evidence suggests that secreted PLA2 group III (sPLA2-III) is associated with colorectal cancer, although its precise role remains obscure. Here we have found that sPLA2-III-null (Pla2g3-/-) mice are highly resistant to colon carcinogenesis. Furthermore, Pla2g3-/- mice are less susceptible to dextran sulfateinduced colitis, implying that the amelioration of colonic inflammation by sPLA2-III ablation may underlie the protective effect against colon cancer. Lipidomics analysis of the colon revealed significant reduction of pro-inflammatory/pro-tumorigenic lysophosholipids as well as unusual elevation of colon-protective fatty acids and their oxygenated metabolites in Pla2g3-/- mice. Overall, our results establish a role of sPLA2-III in the promotion of colorectal inflammation and cancer, expand our understanding of the divergent roles of multiple PLA2 enzymes in the gastrointestinal tract, and point to sPLA2-III as a novel druggable target for colorectal diseases.
Overall design Model, Azoxymethane-induced carcinogenesis. Mice were intraperitoneally administered azoxymethane once a week for 6 weeks and then sacrificed 28 weeks after the last treatment. Condition, tissues with colorectal cancer (AOM) versus normal (vehicle) colon tissues, and Pla2g3 knockout (–/–) versus Pla2g3 wild-type (+/+); Samples, mixed sample of colon tissues from 3-5 mice, respectively
Web link
Contributor(s) Murase R, Taketomi Y, Nishito Y, Murakami M
Citation(s) 28947740
Submission date Aug 08, 2017
Last update date Jan 14, 2024
Contact name Yoshitaka Taketomi
Organization name The University of Tokyo
Department CDBIM, Graduate School of Medicine,
Lab Laboratory of Microenvironmental Metabolic Health Science
Street address 7-3-1 Hongo, Bunkyo-ku,
City Tokyo
ZIP/Postal code 113-8655
Country Japan
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (4)
GSM2735926 Pla2g3+/+ colon_vehicle
GSM2735927 Pla2g3-/- colon_vehicle
GSM2735928 Pla2g3+/+ colon_azoxymethane
BioProject PRJNA397613

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102389_RAW.tar 42.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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