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Series GSE102181 Query DataSets for GSE102181
Status Public on Jan 15, 2020
Title Yap/Taz negatively regulate bone angiogenesis and osteogenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In long bone, the metaphyseal region is less hypoxic than the bone marrow (bone shaft). To study the regional differences of vasculatures, we isolated endothelial cells (ECs) expressing membrane-anchored mTomato protein and nuclear H2B-GFP from Cdh5-mT/nG transgenic reporter femoral metaphysis and diaphyseal bone marrow cavity by fluorescence-activated cell sorting (FACS) at high purity for RNA sequencing (RNA-seq). Next, to study the Hippo signaling in bone ECs, we performed RNA-seq analysis of freshly isolated bone ECs from Cdh5Cre-ERT2-positive Yap1/Taz double floxed or Cdh5Cre-ERT2-positive Lats2 floxed allele and their Cre-negative corresponding littermate controls.
 
Overall design Control and Cdh5(PAC)CreERT2-Yap1; Wwtr1 mutant bone endothelial cells were isolated by using FACS. RNA was isolated from control and EC-Yap1; Wwtr1 mutant endothelial cells.
Bone endothelial cells (Cdh5 mT/nG+) from metaphyseal and marrow transcripts were analyzed. We studied gene expression changes in absence of Yap1/Taz and Lats2 in bone endothelial cells.
 
Contributor(s) Sivaraj KK, Jeong H, Kato K, Adams RH
Citation(s) 31958058
Submission date Aug 02, 2017
Last update date Jul 25, 2021
Contact name Kishor Kumar Sivaraj
E-mail(s) kishor.sivaraj@mpi-muenster.mpg.de
Organization name Max Planck Institute for Molecular Biomedicine
Department Department of Tissue Morphogenesis
Lab Adams lab
Street address Roentgenstrasse 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (16)
GSM2730031 control1
GSM2730032 control2
GSM2730033 control3
Relations
BioProject PRJNA396943
SRA SRP114705

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102181_Lats2_FPKM.txt.gz 521.2 Kb (ftp)(http) TXT
GSE102181_RAW.tar 930.0 Kb (http)(custom) TAR (of TXT)
GSE102181_all_FPKM.txt.gz 1023.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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