|
| Status |
Public on Feb 13, 2018 |
| Title |
Detection of viral double-stranded RNA from Vero cells infected with HSV-1-mCherry |
| Organisms |
Chlorocebus aethiops; Human alphaherpesvirus 1 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The aim of this experiment was to determine whether viral double-stranded RNA from HSV-1 infected cells were detectable in non infected cells
|
| |
|
| Overall design |
Vero cells were infected with mCherry-tagged HSV-1 virus and sorted for mCherry in order to deliniate between infected and uninfected cells. Stranded RNAseq was subsequently performed in order to identify overlapping HSV-1 RNA derived from both strands of the viral genome which correspond to double-stranded RNA.
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| |
|
| Contributor(s) |
Nguen TA, Pang KC, Garnham AL |
| Citation(s) |
28916264 |
| |
| Submission date |
Jul 27, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Alexandra Garnham |
| E-mail(s) |
garnham.a@wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (2) |
| GPL22096 |
Illumina NextSeq 500 (Chlorocebus aethiops) |
| GPL23837 |
Illumina NextSeq 500 (Chlorocebus aethiops; Human alphaherpesvirus 1) |
|
| Samples (9)
|
| GSM2719509 |
HSV-1 infected (negative) vero cells replicate 1 |
| GSM2719510 |
HSV-1 infected (negative) vero cells replicate 2 |
| GSM2719511 |
HSV-1 infected (negative) vero cells replicate 3 |
| GSM2719512 |
HSV-1 infected (positive) vero cells replicate 1 |
| GSM2719513 |
HSV-1 infected (positive) vero cells replicate 2 |
| GSM2719514 |
HSV-1 infected (positive) vero cells replicate 3 |
| GSM2719515 |
Uninfected vero cells replicate 1 |
| GSM2719516 |
Uninfected vero cells replicate 2 |
| GSM2719517 |
Uninfected vero cells replicate 3 |
|
| Relations |
| BioProject |
PRJNA396021 |
| SRA |
SRP113689 |
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