|
| Status |
Public on May 16, 2018 |
| Title |
Genome-wide maps of Tet1 binding in [WT, Tet1-Flag] and [Dnmt3a-/-, Tet1-Flag] J1 ES cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In order to assess Tet1 binding, we first generated a Flag tagged Tet1 ES cells and then knocked out Dnmt3a in the [WT, Tet1-Flag] cells. By Tet1 ChIP and Flag ChIP, we showed that Tet1 binding was complementary to Dnmt3a. And Tet1 binding was not affected or slightly increased at majority of its targets.
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| |
|
| Overall design |
ChIP-seq with anti-Tet1 or anti-Flag in [WT, Tet1-Flag] and [Dnmt3a-/-, Tet1-Flag] ES cells
|
| |
|
| Contributor(s) |
Lin X, Gu T, Li W, Goodell MA |
| Citation(s) |
30001199 |
| |
| Submission date |
Jul 08, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
xueqiu lin |
| E-mail(s) |
xueqiu@fredhutch.org
|
| Organization name |
Stanford University
|
| Street address |
450 Serra Mall
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE100957 |
The role of DNMT3A and TET1 in regulating promoter epigenetic landscapes |
|
| Relations |
| BioProject |
PRJNA393525 |
| SRA |
SRP111380 |
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