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Series GSE100719 Query DataSets for GSE100719
Status Public on Jan 25, 2018
Title Distinct 5-methylcytosine profiles in poly(A)RNA from mouse bone marrow derived mast cells
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Purpose: To transcriptome widely reveal 5-mC regulation by Tet2, we performed bisulfite-sequencing of mRNAs from Tet2-deficient BMMCs and the control cells.Methods: Mouse BMMCs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse IL-3 and SCF. BMMCs were stained to confirm the surface expression of FcɛRI and c-Kit. Cells with purity >97.5% were used for RNA isolation. Results: We carried out an unbiased global analysis of m5C in poly(A)RNA of mouse bone marrow derived mast cells (BMMCs).We show that there were indeed much more mCs in Tet2-deficient group compared with the control, more than half of which located in genic region. Furthermore, we observed that although much less mCs distributed in the three elements of exon regions, more mCs located in 3`-UTR for Tet2-deficient group compared with the control.
Overall design RNA was isolated from Tet2 deficient bone marrow derived mast cells (BMMC) and control cells and RNA-Bisulfite sequencing was performed. We performed two biological replicates and each contain two technical replicates
Contributor(s) Shen Q, Cao X
Citation(s) 29364877
Submission date Jul 03, 2017
Last update date May 15, 2019
Contact name Qian Zhang
Phone +8615921196443
Organization name Second military medical university
Street address xiangyin road 800
City shang hai
ZIP/Postal code 200433
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (8)
GSM2692014 WT-biological replicate 1-technical replicate 1
GSM2692015 WT-biological replicate 1-technical replicate 2
GSM2692016 KO-biological replicate 1-technical replicate 1
BioProject PRJNA392872
SRA SRP110959

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Supplementary file Size Download File type/resource
GSE100719_mC_Tet2_KO.txt.gz 75.0 Kb (ftp)(http) TXT
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