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| Status |
Public on Oct 18, 2017 |
| Title |
Age-dependent human β cell proliferation induced by GLP-1 and calcineurin signaling |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We developed an engraftment strategy to examine age-associated human islet cell replication and found that Ex-4, an agonist of GLP-1R, stimulates human β cell proliferation in juvenile, but not adult islets. We showed that this β cell proliferative response to Ex-4 requires Cn/NFAT signaling.
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| |
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| Overall design |
We transplanted human islets under renal capsule of NSG mice and removed the human islet grafts 4 weeks after Ex-4 or PBS treatment. The total RNAs were extracted from the grafts and used for RNAseq.
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| Contributor(s) |
Powers AC, Dai C |
| Citation(s) |
28920919 |
| Submission date |
Jun 29, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
NRIPESH PRASAD |
| E-mail(s) |
nprasad@hudsonalpha.org
|
| Phone |
2563279413
|
| Organization name |
HUDSONALPHA INSTITUTE FOR BIOTECHNOLOGY
|
| Department |
GENOMIC SERVICES LABORATORY
|
| Street address |
601 GENOME WAY
|
| City |
HUNTSVILLE |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL22245 |
Illumina HiSeq 2500 (Homo sapiens; Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA392445 |
| SRA |
SRP110759 |
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