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Series GSE100616 Query DataSets for GSE100616
Status Public on Sep 22, 2017
Title Gene expression profiling of Pml wt and Pml KO mice liver with acetaminophen (apap) overdose (300mg/kg) i.p.
Organism Mus musculus
Experiment type Expression profiling by array
Summary The Pml gene is essential to the formation of PML nuclear bodies, domains which have been associated with various functions such as apoptosis/senescence, DNA repair and cell proliferation( Lallemand-Breitenbach 2010). PML-NBs formation is regulated by cellular stress including oxidative stress(Jeanne 2010, de The 2012). To investigate the role of PML in ROS response in vivo, we analyse the expression difference to the acetaminophen toxicity, which is initiated by ROS, in Pml wt and Pml KO mice.
Overall design Liver samples were collected from Pml wt and Pml KO untreated or treated with acetaminophen at 2h or 6h time points. 2 samples each. Samples are stabilized in RNAlater and stocked in -20°C. Untreated samples are used as a control. Total RNA samples were extracted using Qiagen kit. Gene expression analysis was done in the plateform of Institute Curie using Affmetrix
Contributor(s) Niwa-Kawakita M, Ferhi O, Soilihi H, Le Bras M, Lallemand-Breitenbach V, de The H
Citation(s) 28931625
Submission date Jun 28, 2017
Last update date Jul 25, 2021
Contact name Michiko Kawakita
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (12)
GSM2688967 Pml wt_ctrl_1
GSM2688968 Pml wt_ctrl_2
GSM2688969 Pml KO_ctrl_1
This SubSeries is part of SuperSeries:
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress
BioProject PRJNA392304

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100616_RAW.tar 50.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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