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Series GSE100615 Query DataSets for GSE100615
Status Public on Sep 22, 2017
Title Gene expression profiling of Pml wt and Pml KO mice liver after fasted 6h
Organism Mus musculus
Experiment type Expression profiling by array
Summary The Pml gene is essential to the formation of PML nuclear bodies, domains which have been associated with various functions such as apoptosis/senescence, DNA repair and cell proliferation( Lallemand-Breitenbach 2010). PML-NBs formation is regulated by cellular stress including oxidative stress(Jeanne 2010, de The 2012). To investigate the role of PML in ROS response in vivo, we analyse the expression difference betweem Pml wt and Pml KO under fasted condition, which easily up-regulate ROS in BALB/cByJ background
Overall design Liver samples were collected from Pml wt and Pml KO ad libitum(2 each) or fasted for 6 hours(3 each). Samples are stabilized in RNAlater and stored in -20°C. Two ad libitum samples are used as a control. Fasted samples are 3 each genotype. Total RNA samples were extracted using QIagen kit. Gene expression analysis was done in the plateform of Institute Curie using Affmetrix
Contributor(s) Niwa-Kawakita M, Ferhi O, Soilihi H, Le Bras M, Lallemand-Breitenbach V, de The H
Citation(s) 28931625
Submission date Jun 28, 2017
Last update date Jul 25, 2021
Contact name Michiko Kawakita
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
Platforms (1)
GPL17400 [MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version]
Samples (10)
GSM2688957 Pml wt_ad libitum 1
GSM2688958 Pml wt_ad libitum 2
GSM2688959 Pml ko_ad libitum 1
This SubSeries is part of SuperSeries:
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress
BioProject PRJNA392303

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100615_RAW.tar 44.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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