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Series GSE100574 Query DataSets for GSE100574
Status Public on Jun 28, 2017
Title Global transcriptomic analyses of bronchial epithelial cells exposed to 5 ng/mL TGF-β1 and 10 nM Estrogen individually and in combination
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To begin to identify downstream consequences of TGF-β1-induced reduction of estrogen receptor expression, we employed RNA-Seq. Results of global transcriptomic analysis showed that TGF-β1 and E2 inversely modulated the expression of several genes involved in both known pro-fibrotic cellular processes such as extracellular matrix turnover and newly identified events including airway smooth muscle cell contraction and calcium flux regulation. We also report, for the first time, that E2 specifically modulated the expression of genes involved in chromatin remodeling pathways and that this regulation was absent in the presence of TGF-β1. Collectively, these results suggest that E2 influences pathways relevant to pulmonary fibrosis and highlights potential roles for E2 in the lung that may contribute to sex-specific differences in IPF.
Overall design Four treatment groups were analyzed; cells exposed to vehicle control (n = 5), 5 ng/mL TGF-β1 (n = 5), 10 nM Estrogen (n = 3), or 5 ng/mL TGF-β1 and 10 nM Estrogen in combination (n = 4)
Contributor(s) Smith LC, Sabo-Attwood T
Citation(s) 30165855
NIH grant(s)
Grant ID Grant title Affiliation Name
UL1 TR001427 Together: Transforming and Translating Discovery to Improve Health UNIVERSITY OF FLORIDA David R Nelson
R01 HL114907 Contribution of Toll-like Receptors in the Pulmonary Response to Nanoparticles an UNIVERSITY OF FLORIDA Tara L Sabo-Attwood
Submission date Jun 27, 2017
Last update date May 15, 2019
Contact name Ley Cody Smith
Phone 4074510956
Organization name University of Florida
Street address 2187 Mowry Rd
City Gainesville
State/province FL
ZIP/Postal code 32611
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (17)
GSM2687353 9: Vehicle Control
GSM2687354 10: Vehicle Control
GSM2687355 11: Vehicle Control
BioProject PRJNA392160
SRA SRP110620

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Supplementary file Size Download File type/resource
GSE100574_allExpressions.csv.gz 2.9 Mb (ftp)(http) CSV
GSE100574_genes.rawmatrix.csv.gz 1.3 Mb (ftp)(http) CSV
GSE100574_transcripts.rawmatrix.csv.gz 4.9 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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