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| Status |
Public on Jun 28, 2017 |
| Title |
Global transcriptomic analyses of bronchial epithelial cells exposed to 5 ng/mL TGF-β1 and 10 nM Estrogen individually and in combination |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To begin to identify downstream consequences of TGF-β1-induced reduction of estrogen receptor expression, we employed RNA-Seq. Results of global transcriptomic analysis showed that TGF-β1 and E2 inversely modulated the expression of several genes involved in both known pro-fibrotic cellular processes such as extracellular matrix turnover and newly identified events including airway smooth muscle cell contraction and calcium flux regulation. We also report, for the first time, that E2 specifically modulated the expression of genes involved in chromatin remodeling pathways and that this regulation was absent in the presence of TGF-β1. Collectively, these results suggest that E2 influences pathways relevant to pulmonary fibrosis and highlights potential roles for E2 in the lung that may contribute to sex-specific differences in IPF.
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| Overall design |
Four treatment groups were analyzed; cells exposed to vehicle control (n = 5), 5 ng/mL TGF-β1 (n = 5), 10 nM Estrogen (n = 3), or 5 ng/mL TGF-β1 and 10 nM Estrogen in combination (n = 4)
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| |
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| Contributor(s) |
Smith LC, Sabo-Attwood T |
| Citation(s) |
30165855 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| UL1 TR001427 |
Together: Transforming and Translating Discovery to Improve Health |
UNIVERSITY OF FLORIDA |
David R Nelson |
| R01 HL114907 |
Contribution of Toll-like Receptors in the Pulmonary Response to Nanoparticles an |
UNIVERSITY OF FLORIDA |
Tara L Sabo-Attwood |
|
| Submission date |
Jun 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Ley Cody Smith |
| E-mail(s) |
leycodysmith@gmail.com
|
| Phone |
4074510956
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| Organization name |
University of Florida
|
| Street address |
2187 Mowry Rd
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32611 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA392160 |
| SRA |
SRP110620 |
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