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Series GSE10040 Query DataSets for GSE10040
Status Public on Nov 10, 2008
Title Expression data from PBMC treated with rabbit anti-thymocyte globulin (rATG) or horse ATG (hATG)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We performed microarray to compare gene expression patterns of PBMC treated with rATG or hATG. Fold changes were compared using 2-way ANOVA tests for untreated, rATG- and hATG-treated PBMC. In PBMC treated with 10 ug/mL rATG, compared with untreated PBMC, 478 genes showed up-regulation, and 341 genes showed down-regulation at 24 hours using 10% FDR and 2-fold change cutoff. Immediately striking was that 10 ug/mL hATG had affected many fewer genes than did rATG: only 3 genes were up-regulated and 6 genes were down-regulated at 24 hours in hATG-treated PBMC. When we compared rATG with hATG, rATG induced up-regulation of 268 genes and down-regulation of 95 genes. These genes belong to the categories of immune response (64 genes), cytokine-cytokine receptor interaction (36 genes), regulation of cell proliferation (24 genes), cell cycle (23 genes), cell growth (8 genes), apoptosis (7 genes), and others.
Keywords: different treatment
Overall design PBMC from healthy controls were cultured with rATG or hATG (each at 10 ug/mL) in 10% FCS/RPMI 1640 at 37C for 24 hours, PBMC without drugs were used as controls. Total RNA was extracted with the RNeasy kit (Qiagen, Valencia, CA). RNA purity was assessed by spectrophotometry. Probes were prepared using standard Affymetrix protocols and hybridized to Affymetrix HG-U133A 2.0 arrays (Affymetrix, Santa Clara, CA)
Contributor(s) Feng X, Xu X, Raghavachari N, Munson PJ, Young NS
Citation(s) 18250226
Submission date Dec 28, 2007
Last update date Dec 06, 2018
Contact name Xingmin Feng
Organization name National Institutes of Health
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (33)
GSM259205 Untreated for 24h, biological rep1 (S1)
GSM259206 Untreated for 24h, biological rep2 (S2)
GSM259207 Untreated for 24h, biological rep3 (S3)
BioProject PRJNA108607

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Supplementary file Size Download File type/resource
GSE10040_RAW.tar 67.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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