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Series GSE100374 Query DataSets for GSE100374
Status Public on Mar 22, 2018
Title HIV-1 infection of human monocyte-derived dendritic cells with and without the integrase inhibitor, Raltegravir (gene expression).
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Myeloid dendritic cells (DCs) have the innate capacity to sense pathogens and orchestrate immune responses. However, DCs do not mount efficient immune responses to HIV-1, due to potent restriction at the level of reverse transcription. Here, we uncover that when reverse transcription is allowed to proceed, DCs detect HIV-1 in distinct phases, before and after integration. Blocking integration suppressed, but did not abolish, activation of the transcription factor, IRF3, interferon responses, and DC maturation. The cytoplasmic DNA sensor, cGAS, and the E3 ligase, TRIM5, were both required for these responses. Consistent with two stages of innate activation, HIV-1 altered host chromatin accessibility before and after integration. Our studies support the hypothesis that HIV-1 replication increases the amount of material available for sensing and that cGAS signaling can be tuned by discrete inflammatory inputs to drive DC maturation.
Overall design DCs were derived from the peripheral blood from two deidentified human donors and infected with a single-cycle HIV-1 reporter virus (HIV-GFP) in the presence of Vpx, with and without Raltegravir. RNA expression was measured at 0, 8, 16, 20, 24, 28, 32, and 40 hours after infection.
Contributor(s) Johnson JS, Amon LM, Aderem A
Citation(s) 29544097
Submission date Jun 22, 2017
Last update date Jul 25, 2021
Contact name Jarrod S Johnson
Organization name Center for Infectious Disease Research
Street address 307 Westlake Ave, Suite 500
City Seattle
State/province Washington
ZIP/Postal code 98109
Country USA
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (32)
GSM2679702 D186_VLP_mock
GSM2679703 D187_VLP_mock
GSM2679704 D186_HIV_8h
This SubSeries is part of SuperSeries:
GSE100377 HIV-1 infection of human monocyte-derived dendritic cells with and without the integrase inhibitor, Raltegravir
BioProject PRJNA391548

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100374_RAW.tar 391.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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