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| Status |
Public on Sep 14, 2017 |
| Title |
Long non-coding RNA HOTAIR promotes cell migration by upregulating insulin growth factor-binding protein 2 in renal cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Renal cell carcinoma (RCC) is one of the most lethal urologic cancers. About one-third of RCC patients already have distal metastasis at the time of diagnosis. There is growing evidence that Hox antisense intergenic RNA (HOTAIR) plays essential roles in metastasis in several types of cancers. However, the precise mechanism by which HOTAIR enhances malignancy remains unclear, especially in RCC. Here, we demonstrated that HOTAIR enhances RCC-cell migration by regulating the insulin growth factor-binding protein 2 (IGFBP2) expression. HOTAIR expression in tumors was significantly correlated with nuclear grade, lymph-node metastasis, and lung metastasis. High HOTAIR expression was associated with a poor prognosis in both our dataset and The Cancer Genome Atlas dataset. Migratory capacity was enhanced in RCC cell lines in a HOTAIR-dependent manner. HOTAIR overexpression accelerated tumorigenicity and lung metastasis in immunodeficient mice. Microarray analysis revealed that IGFBP2 expression was upregulated in HOTAIR-overexpressing cells compared with control cells. The enhanced migration activity of HOTAIR-overexpressing cells was attenuated by IGFBP2 knockdown. IGFBP2 and HOTAIR were co-expressed in clinical RCC samples. Our findings suggest that the HOTAIR-IGFBP2 axis plays critical roles in RCC metastasis and may serve as a novel therapeutic target for advanced RCC.
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| Overall design |
HOTAIR-overexpressing (HOTAIR1-3) and control (EV1-4) ACHN cells
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| Contributor(s) |
Katayama H, Tamai K, Shibuya R, Nakamura M, Mochizuki M, Yamaguchi K, Kawamura S, Tochigi T, Sato I, Okanishi T, Sakurai K, Fujibuchi W, Arai Y, Satoh K |
| Citation(s) |
28931862 |
| Submission date |
Jun 16, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Keiichi Tamai |
| E-mail(s) |
tamaikeiichi@med.tohoku.ac.jp
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| Organization name |
Miyagi Cancer Center
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| Street address |
1-47, Nodayama, Shiote, Medeshima
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| City |
Natori, Miyagi |
| ZIP/Postal code |
981-1293 |
| Country |
Japan |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA390728 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100107_RAW.tar |
89.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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