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Series GSE100055 Query DataSets for GSE100055
Status Public on Dec 06, 2017
Title Prep1 prevents premature adipogenesis of mesenchymal progenitors [ChIP-seq]
Organism Mus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Transcriptional regulators are crucial in adipocyte differentiation. We now show that the homeodomain-containing transcription factor Prep1 is a repressor of adipogenic differentiation since its down-regulation (DR) in both ex vivo bone marrow-derived mesenchymal stromal cells (MSC) and in vitro 3T3-L1 pre-adipocytes significantly increases their adipogenic differentiation ability. Prep1 acts at a stage preceding the activation of the differentiation machinery because its DR makes cells more prone to adipogenic differentiation even in the absence of the adipogenic inducers. Prep1 DR expands the DNA binding landscape of C/EBPß(CAAT enhancer binding protein ß) without affecting its expression or activation. The data indicate that Prep1 normally acts by restricting DNA binding of transcription factors to adipogenic enhancers, in particular C/EBPß.
 
Overall design ChIP-seq performed using Cebpß Ab
 
Contributor(s) Penkov D, Morelli M
Citation(s) 29138456
Submission date Jun 15, 2017
Last update date May 15, 2019
Contact name Marco J. Morelli
E-mail(s) Marco.Morelli@iit.it
Organization name Istituto Italiano di Tecnologia
Department Center for Genomic Science of IIT@SEMM
Street address via Adamello 16
City Milan
ZIP/Postal code 20139
Country Italy
 
Platforms (1)
GPL23584 Ion Torrent Proton (Mus)
Samples (8)
GSM2668828 Control cells -2days ChIP
GSM2668829 Prep1down cells -2days ChIP
GSM2668830 Control cells 4hours ChIP
This SubSeries is part of SuperSeries:
GSE100057 Prep1 prevents premature adipogenesis of mesenchymal progenitors
Relations
BioProject PRJNA390578
SRA SRP109254

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100055_Control_-2d.narrowPeak.gz 20.5 Kb (ftp)(http) NARROWPEAK
GSE100055_Control_4h.narrowPeak.gz 306.0 Kb (ftp)(http) NARROWPEAK
GSE100055_Prep1down_-2d.narrowPeak.gz 39.6 Kb (ftp)(http) NARROWPEAK
GSE100055_Prep1down_4h.narrowPeak.gz 625.0 Kb (ftp)(http) NARROWPEAK
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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