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Status |
Public on Dec 06, 2017 |
Title |
Prep1 prevents premature adipogenesis of mesenchymal progenitors [ChIP-seq] |
Organism |
Mus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Transcriptional regulators are crucial in adipocyte differentiation. We now show that the homeodomain-containing transcription factor Prep1 is a repressor of adipogenic differentiation since its down-regulation (DR) in both ex vivo bone marrow-derived mesenchymal stromal cells (MSC) and in vitro 3T3-L1 pre-adipocytes significantly increases their adipogenic differentiation ability. Prep1 acts at a stage preceding the activation of the differentiation machinery because its DR makes cells more prone to adipogenic differentiation even in the absence of the adipogenic inducers. Prep1 DR expands the DNA binding landscape of C/EBPß(CAAT enhancer binding protein ß) without affecting its expression or activation. The data indicate that Prep1 normally acts by restricting DNA binding of transcription factors to adipogenic enhancers, in particular C/EBPß.
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Overall design |
ChIP-seq performed using Cebpß Ab
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Contributor(s) |
Penkov D, Morelli M |
Citation(s) |
29138456 |
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Submission date |
Jun 15, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Marco J. Morelli |
E-mail(s) |
Marco.Morelli@iit.it
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Organization name |
Istituto Italiano di Tecnologia
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Department |
Center for Genomic Science of IIT@SEMM
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Street address |
via Adamello 16
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City |
Milan |
ZIP/Postal code |
20139 |
Country |
Italy |
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Platforms (1) |
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Samples (8)
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This SubSeries is part of SuperSeries: |
GSE100057 |
Prep1 prevents premature adipogenesis of mesenchymal progenitors |
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Relations |
BioProject |
PRJNA390578 |
SRA |
SRP109254 |