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Platform GPL4680 Query DataSets for GPL4680
Status Public on Mar 19, 2007
Title Eurogentec S. pombe ORF array (B130F)
Technology type spotted DNA/cDNA
Distribution custom-commercial
Organism Schizosaccharomyces pombe
Manufacturer Eurogentec AG
Manufacture protocol Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). We normally achieve a success rate of 93 % of PCR reactions. For the failed PCR reactions we perform three different protocols of PCR conditions, which include different MgCl2 concentrations and different Taq polymerase. We should normally get an additional 2 % working and reach a success rate of 95 % in total. We check 5 % of the total oligos by Mass Spectrometry and will re-synthesize maximum 2 % of the oligos based on a newly designed primer. We will perform the PCR reactions with the new designed oligonucleotides with the standard PCR protocol. Following this process we reach usually 97 % success rate.
Secondary PCR: amplifications will be performed using a single pair of primers annealing to tag sequences introduced on primary PCR primers. These PCR amplifications will also be controlled by agarose gel electrophoresis and repeated if required reaching a minimum of 95 % success rate. The final PCR products will contain an amino group at their 5' end to promote the attachment of the predicted gene coding DNA strand to the treated glass slides.
 
 
Contributor(s) Ekwall K, Wright T, Talibi D, Haas SA
Submission date Dec 19, 2006
Last update date Mar 19, 2007
Contact name Karl Ekwall
E-mail(s) karl.ekwall@ki.se
Phone +46 8 6089133
Organization name Karolinska Inst
Street address Alfred Nobels Alle 7
City Stockholm
ZIP/Postal code S-141 89
Country Sweden
 
Samples (4) GSM151849, GSM151850, GSM151851, GSM151852
Series (1)
GSE6557 A genome wide role for CHD remodeling factors and Nap1 in nucleosome disassembly

Data table header descriptions
ID
Block block
Column column
Row row
ORF Gene name
SPOT_ID

Data table
ID Block Column Row ORF SPOT_ID
1 1 1 1 SPBC215.09c
2 1 2 1 SPBC215.09c
3 1 3 1 vti1
4 1 4 1 vti1
5 1 5 1 SPBC3B9.21
6 1 6 1 SPBC3B9.21
7 1 7 1 E coli 2
8 1 8 1 E coli 2
9 1 9 1 E coli 1
10 1 10 1 E coli 1
11 1 11 1 Ef1-b
12 1 12 1 Ef1-b
13 1 13 1 Buffer
14 1 14 1 Buffer
15 1 15 1 Empty
16 1 16 1 Empty
17 1 17 1 Empty
18 1 18 1 Empty
19 1 19 1 Empty
20 1 20 1 Empty

Total number of rows: 22176

Table truncated, full table size 582 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided

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