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Status
Public on Dec 22, 2006
Title
University of London Bacterial Microarray Group at St George's S. pneumoniae 2.3K
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Streptococcus pneumoniae
Manufacturer
The Bacterial Microarray Group at St George's, University of London (BµG@S)
Manufacture protocol
See the website of The Bacterial Microarray Group at St George's, University of London
Description
The S.pneumoniae array was designed using the TIGR4 strain as a base strain with annotation described in Tettelin et al (2001) Science 293: 498-506. PCR primers were designed and products ampilifed to 2236 target genes. The genes extra to strain R6 where identified from the annotation described in Hoskins et al (2001) J. Bacteriol. 183: 5709-5717. PCR primers were thus designed to 117 R6 target genes and added to the array
Submission date
Jul 16, 2006
Last update date
Dec 22, 2006
Contact name
Alistair Standish
E-mail(s)
alistair.standish@adelaide.edu.au
Organization name
University of Adelaide
Department
School of Molecular and Biomedical Sciences
Street address
North Terrace
City
Adelaide
State/province
S.A.
ZIP/Postal code
5005
Country
Australia
Samples (28)
GSM120590 , GSM120607 , GSM120608 , GSM120609 , GSM120805 , GSM120806
GSM120807 ,
GSM120808 ,
GSM120809 ,
GSM120810 ,
GSM1888857 ,
GSM1888858 ,
GSM1888859 ,
GSM1888860 ,
GSM1888861 ,
GSM1888862 ,
GSM1888863 ,
GSM1888864 ,
GSM1888865 ,
GSM1888866 ,
GSM1888867 ,
GSM1888868 ,
GSM1888869 ,
GSM1888870 ,
GSM1888871 ,
GSM1888872 ,
GSM1888873 ,
GSM1888874
Series (3)
GSE5322
Investigation of pneumococcal two-component signal transduction system RR/HK06 in D39
GSE5330
Investigation of pneumococcal two-component signal transduction system RR/HK06 in TIGR4
GSE73217
Characterization of Pneumococcal Genes Involved in Bloodstream Invasion in a Mouse Model
Data table header descriptions
ID
Block
Microarray block index
Column
Microarray column index
Row
Microarray row index
SP ID
Sp identification number. SpTIGR4 = TIGR4 sequence (Tettelin et al (2001) Science 293: 498-506). SpR6 = R6 Sequence ( Hoskins et al (2001) J. Bacteriol. 183: 5709-5717.)
ORF
SPOT_ID
Data table
ID
Block
Column
Row
SP ID
ORF
SPOT_ID
1
1
1
1
111Cy3 lights (8A12)
111Cy3 lights (8A12)
2
1
1
2
112SC-Calibration_01 (9A4)
112SC-Calibration_01 (9A4)
3
1
1
3
113SpTIGR4-2144 (6E16)
Sp2144
4
1
1
4
114SpTIGR4-1980 (6A8)
Sp1980
5
1
1
5
115SpTIGR4-1820 (5A24)
Sp1820
6
1
1
6
116SpTIGR4-1728 (5M12)
Sp1728
7
1
1
7
117SpTIGR4-1664 (5M4)
Sp1664
8
1
1
8
118SpTIGR4-1500 (4I20)
Sp1500
9
1
1
9
119SpTIGR4-1340 (4I12)
Sp1340
10
1
1
10
1110SpTIGR4-1280 (4M4)
Sp1280
11
1
1
11
1111SpTIGR4-1119 (3M20)
Sp1119
12
1
1
12
1112SpTIGR4-0863 (3E12)
Sp0863
13
1
1
13
1113SpTIGR4-0795 (3A4)
Sp0795
14
1
1
14
1114SpTIGR4-0699 (2I16)
Sp0699
15
1
1
15
1115SpTIGR4-0445 (2E8)
Sp0445
16
1
1
16
1116SpTIGR4-0349 (1M20)
Sp0349
17
1
1
17
1117SpTIGR4-0185 (1I12)
Sp0185
18
1
1
18
1118SpTIGR4-0025 (1A4)
Sp0025
19
1
1
19
1119Cy5 lights (8E12)
1119Cy5 lights (8E12)
20
1
2
1
12150% DMSO (7M12)
12150% DMSO (7M12)
Total number of rows: 5776 Table truncated, full table size 267 Kbytes .
Supplementary data files not provided