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Platform GPL3433 Query DataSets for GPL3433
Status Public on Feb 08, 2006
Title NADC MAP Oligo v1.0
Technology type spotted oligonucleotide
Distribution non-commercial
Organisms Mycobacterium avium subsp. paratuberculosis; Mycobacterium avium subsp. avium
Manufacturer National Animal Disease Center, USDA-ARS
Manufacture protocol 70mer oligos were designed using ArrayOligoSelector. Input sequences included all annotated ORFs and intergenic regions > 500 bp from MAP K10 as well as all MAA 104 ORFs with < 30% identity to MAP K10. MAA 104 sequence was obtained from TIGR on 07/09/04 and automatically annotated using Reganor software (McHardy et al). One oligo was designed for each ORF < 2000 bp and two oligos were designed for each ORF > 2000 bp. Six Arabidopsis clones and MAP K10 sheared genomic DNA are included as controls. Oligos were synthesized by Illumina. The microarray was printed using a BioRobotics arrayer equiped with 48 quill tip pins. Each source well was printed in triplicate on the array.
Support glass
Coating other
 
 
Contributor(s) Paustian ML, Bannantine JP, Kapur V
Submission date Feb 07, 2006
Last update date Apr 01, 2008
Contact name Mike Paustian
E-mail(s) mpaustia@nadc.ars.usda.gov
Organization name National Animal Disease Center, USDA-ARS
Street address 2300 Dayton Ave
City Ames
State/province IA
ZIP/Postal code 50010
Country USA
 
Samples (161) GSM172202, GSM172203, GSM172204, GSM172205, GSM172206, GSM172207 
Series (2)
GSE7622 Comparative genomic hybridizations of Mycobacterium avium isolates obtained from multiple host species
GSE62566 Characterization of the inflammatory phenotype of Mycobacterium avium subspecies paratuberculosis

Data table header descriptions
ID
sa Array Row
sa Array Column
sa Spot Row
sa Spot Column
gpr Block
gpr Column
gpr Row
PlateID
SPOT_ID Spot identification - usually the ORF number
GB_ACC Genbank accession number (if available)
GENOME_ACC Accession.version[ORF_START..ORF_END]
ORGANISM Organism that the sequence was annotated from
ORF
ORF_START ORF start coordinates
ORF_END ORF end coordinates
COMMENT Additional information regarding the oligos
PT_GI_LIST Genbank GI designation (if available)

Data table
ID sa Array Row sa Array Column sa Spot Row sa Spot Column gpr Block gpr Column gpr Row PlateID SPOT_ID GB_ACC GENOME_ACC ORGANISM ORF ORF_START ORF_END COMMENT PT_GI_LIST
1 1 1 1 1 1 1 1 13I24 MAP3721 AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP3721 4140311 4141786 41409819
2 1 1 1 2 1 2 1 13M24 MAP3816 AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP3816 4264949 4265191 41409914
3 1 1 1 3 1 3 1 14A12 MAP3893c_5p AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP3893c 4356884 4359175 41409991
4 1 1 1 4 1 4 1 14E12 MAP3985c AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP3985c 4443184 4444128 41410083
5 1 1 1 5 1 5 1 14I12 MAP4077c_3p AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP4077c 4544877 4547252 41410175
6 1 1 1 6 1 6 1 14M12 MAP4168 AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP4168 4642379 4642795 41410266
7 1 1 1 7 1 7 1 14A24 MAP3904 AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP3904 4367269 4367601 41410002
8 1 1 1 8 1 8 1 14E24 MAP3997c AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP3997c 4454110 4455021 41410095
9 1 1 1 9 1 9 1 14I24 MAP4089 AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP4089 4559674 4561212 41410187
10 1 1 1 10 1 10 1 14M24 MAP4179 AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP4179 4652705 4653268 41410277
11 1 1 1 11 1 11 1 15A12 MAP4261 AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP4261 4730626 4731252 41410359
12 1 1 1 12 1 12 1 15E12 AY056187 AY056187 Arabidopsis thaliana Clones corresponding to the Arabidopsis controls can be ordered from the RIKEN or TAIR public repositories
13 1 1 1 13 1 13 1 emp -- empty
14 1 1 1 14 1 14 1 emp -- empty
15 1 1 1 15 1 15 1 15A24 MAP4273c AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP4273c 4740921 4741229 41410371
16 1 1 1 16 1 16 1 15E24 MAP4350c AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP4350c 4829033 4829176 41410448
17 1 1 1 17 1 17 1 emp -- empty
18 1 1 1 18 1 18 1 emp -- empty
19 1 1 1 19 1 19 1 emp -- empty
20 1 1 2 1 1 1 2 11M12 MAP3065 AE016958.1 Mycobacterium avium subsp. paratuberculosis MAP3065 3410686 3411987 41409163

Total number of rows: 17328

Table truncated, full table size 2688 Kbytes.






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