|
| Status |
Public on May 03, 2006 |
| Title |
Eurogentec AG S. pombe ORF array 10k (1st batch) |
| Technology type |
spotted DNA/cDNA |
| Distribution |
custom-commercial |
| Organism |
Schizosaccharomyces pombe |
| Manufacturer |
Eurogentec AG |
| Manufacture protocol |
Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). We normally achieve a success rate of 93 % of PCR reactions. For the failed PCR reactions we perform three different protocols of PCR conditions, which include different MgCl2 concentrations and different Taq polymerase. We should normally get an additional 2 % working and reach a success rate of 95 % in total. We check 5 % of the total oligos by Mass Spectrometry and will re-synthesize maximum 2 % of the oligos based on a newly designed primer. We will perform the PCR reactions with the new designed oligonucleotides with the standard PCR protocol. Following this process we reach usually 97 % success rate.
|
| Support |
glass |
| |
|
| Description |
4976 ORFs spotted in duplicate on glass slide
Keywords = yeast ORF DNA microarray
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| |
|
| Web link |
http://www.eurogentec.com/
|
| Submission date |
Feb 03, 2006 |
| Last update date |
May 03, 2006 |
| Contact name |
Anthony Wright |
| E-mail(s) |
yongtao.xue.franzen@ki.se
|
| Organization name |
Karolinska Institute
|
| Lab |
Anthony Wright
|
| Street address |
Blickagången 6
|
| City |
Huddinge |
| ZIP/Postal code |
141 57 |
| Country |
Sweden |
| |
|
| Samples (6) |
GSM118250, GSM118251, GSM118252, GSM118253, GSM118254, GSM118255 |
| Series (3) |
| GSE5218 |
Comparison of gene regulation by Gcn5 under KCl stress in S. cerevisiae and S. pombe |
| GSE5227 |
Stress-specific role of the fission yeast Gcn5 acetylatransferase in programming a subset of stress-response genes |
| GSE16556 |
Role of Gcn5 in KCl stress adaptation in S. cerevisiae and S. pombe: transcriptional and genomic analyses |
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