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Platform GPL11158 Query DataSets for GPL11158
Status Public on Jan 26, 2011
Title 021301-MLU Fusarium graminearum 8x15K v1.0
Technology type in situ oligonucleotide
Distribution custom-commercial
Organism Fusarium graminearum
Manufacturer Agilent Technologies
Manufacture protocol The microarray is manufactured by Agilent using its proprietary SurePrint inkjet technogy that synthesizes 60mer oligonucleotides onto specially-prepared glass slides. For detailed information see manufacturer's web site
Description The microarray was designed in collaboration with an Agilent-certified service provider (imaGenes, Berlin, Germany) that applied an in-house developed approach for selection of the best performing probes for each gene ( Using the F. g. genome annotation v3.0 annotation (FG3), for each gene model, several 60mer oligonucleotides were selected by the software eArray ( and were then filtered by proprietary software (imaGenes). In addition to the 13,332 genes of the F. g. genome annotation v3.0, available cDNA sequences of the TRI (= trichothecene biosynthesis) gene cluster from nivalenol (NIV) producing strains of F. graminearum were chosen for oligonucleotide selection. Several suitable oligonucleotides per gene were assembled on a pre-selection array (2x105K format) and hybridized against Cy3-labeled genomic DNA and cRNA (from a pooled RNA sample), respectively. Based on the RNA hybridization results, for each gene the relative ratio of probes was determined that yielded significant signal intensities. In case that this ratio was 60% higher than the background, a gene was considered expressed. For these genes, final oligonucleotide selection relied on the results from RNA hybridization, whereas for genes considered non-expressed hybridization results from genomic DNA were used. The final 15K array design comprises 15,744 features (15,208 F. g. - specific oligos + 536 internal controls). 11,476 genes and 10 TRI cluster ESTs were represented by one oligonucleotide, 1,854 genes and 7 TRI cluster ESTs two probes were chosen.
Web link
Contributor(s) Becher R, Wirsel SG
Submission date Nov 03, 2010
Last update date Jan 26, 2011
Contact name Rayko Becher
Organization name Martin-Luther-University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences
Department Department of Phytopathology and Plant Protection
Street address Betty-Heimann-Str. 3
City Halle/Saale
ZIP/Postal code 06120
Country Germany
Samples (6) GSM617106, GSM617107, GSM617108, GSM617109, GSM617110, GSM617111
Series (1)
GSE25114 Genome-wide expression profiling of Fusarium graminearum in response to azole fungicide treatment

Data table header descriptions

Data table
FGSG_00001 FGSG_00001
FGSG_00002 FGSG_00002
FGSG_00003 FGSG_00003
FGSG_00004 FGSG_00004
FGSG_00005 FGSG_00005
FGSG_00006 FGSG_00006
FGSG_00007 FGSG_00007
FGSG_00009 FGSG_00009
FGSG_00010 FGSG_00010
FGSG_00011 FGSG_00011
FGSG_00012 FGSG_00012
FGSG_00013 FGSG_00013
FGSG_00014 FGSG_00014
FGSG_00015 FGSG_00015
FGSG_00016 FGSG_00016
FGSG_00017 FGSG_00017
FGSG_00018 FGSG_00018
FGSG_00019 FGSG_00019
FGSG_00020 FGSG_00020
FGSG_00021 FGSG_00021

Total number of rows: 13330

Table truncated, full table size 286 Kbytes.

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary file Size Download File type/resource
GPL11158_full_platform.txt.gz 499.4 Kb (ftp)(http) TXT

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