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| Status |
Public on Mar 08, 2019 |
| Title |
Gene expression profiling of planarians after inhibition of hmcn-1 |
| Organism |
Schmidtea mediterranea |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Inhibition of hemicentin-1, which encodes a highly conserved ECM glycoprotein specifically expressed in muscle, leads to aberrant localization of internal parenchymal cells, including planarian stem cells (neoblasts) and differentiated cells, between the outer epidermis and sub-epidermal muscle.
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| Overall design |
Polyadenylated RNA was isolated from tail fragments planarians following feeding with in vitro transcribed RNAi of a control gene (C.elegans unc-22; 3 replicates) or hmcn-1 (3 replicates). Worms were fed four times every other day and cut 5 days after final feeding for a total of 13 days, a timepoint before ectopic cells are visible.
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| Contributor(s) |
Cote LE, Reddien PW |
| Citation(s) |
30962434 |
| |
| Submission date |
Sep 12, 2018 |
| Last update date |
Apr 17, 2019 |
| Contact name |
Peter W Reddien |
| Organization name |
Whitehead Institute for Biomedical Research
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL15553 |
Illumina HiSeq 2000 (Schmidtea mediterranea) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA490440 |
| SRA |
SRP161555 |
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