Naive PDGF-B library

Wildtype PDGF-B: Control for sequencing errors

PDGF-B library sorted for weak or absent binding to 30 nM PDGFRbeta-Fc

PDGF-B library sorted for high binding to 30 nM PDGFRbeta-Fc

PDGF-B library sorted for weak or absent binding to 50 nM PDGFRbeta-Fc

PDGF-B library sorted for high binding to 50 nM PDGFRbeta-Fc

Naïve library of S variants

SARS-CoV-2 S-RBD library sorted for high binding to human sACE2 (wild type)

SARS-CoV-2 S-RBD library sorted for low binding to human sACE2 (wild type)

SARS-CoV-2 S-RBD library sorted for increased binding specificity towards wild type sACE2 (Independent Rep 1)

SARS-CoV-2 S-RBD library sorted for increased binding specificity towards wild type sACE2 (Independent Rep 2)

SARS-CoV-2 S-RBD lib sorted for increased binding specificity towards the engineered sACE2.v2.4 (Independent Rep 1)

SARS-CoV-2 S-RBD lib sorted for increased binding specificity towards the engineered sACE2.v2.4 (Independent Rep 2)

Library of tapasin variants

Tapasin library sorted for rescue of surface HLA-A2 expression in tapasin-KO cells (Replicate 1)

Tapasin library sorted for rescue of surface HLA-A2 expression in tapasin-KO cells (Replicate 2)

Naive PDGFRA-D2D3 library

UL74-High Replicate1

UL74-High Replicate2

UL74-Low Replicate1