| Blue light is a universal signal for Escherichia coli chemoreceptors. | Blue Light Is a Universal Signal for Escherichia coli Chemoreceptors.
Perlova T, Gruebele M, Chemla YR., Free PMC Article | 04/4/2020 |
| Thus, the proportion of polypeptide chain that is locally and presumably transiently disordered is a structural feature of cytoplasmic domain dynamics that varies with functional region and modification-induced signaling state. | Bacterial Chemoreceptor Dynamics: Helical Stability in the Cytoplasmic Domain Varies with Functional Segment and Adaptational Modification.
Bartelli NL, Hazelbauer GL., Free PMC Article | 06/24/2017 |
| There is a differential repositioning of the second transmembrane helices from E. coli Tar and EnvZ upon moving the flanking aromatic residues. | Differential repositioning of the second transmembrane helices from E. coli Tar and EnvZ upon moving the flanking aromatic residues.
Botelho SC, Enquist K, von Heijne G, Draheim RR., Free PMC Article | 09/5/2015 |
| the Tar(FO) modules demonstrate that trimerized signaling tips self-associate, bind CheA and CheW, and facilitate conversion of CheA to an active conformation. | Preformed Soluble Chemoreceptor Trimers That Mimic Cellular Assembly States and Activate CheA Autophosphorylation.
Greenswag AR, Li X, Borbat PP, Samanta D, Watts KJ, Freed JH, Crane BR., Free PMC Article | 08/22/2015 |
| Residues at the cytoplasmic end of transmembrane helix 2 determine the signal output of the Tar chemoreceptor. | Residues at the cytoplasmic end of transmembrane helix 2 determine the signal output of the TarEc chemoreceptor.
Adase CA, Draheim RR, Rueda G, Desai R, Manson MD. | 06/29/2013 |
| Such inversion is enabled by opposing pH sensing by the two major chemoreceptors, Tar and Tsr, such that the relative strength of the response is modulated by adaptive receptor methylation. | Opposite responses by different chemoreceptors set a tunable preference point in Escherichia coli pH taxis.
Yang Y, Sourjik V. | 06/1/2013 |
| Here we report the overexpression system for aCB2 in Escherichia coli C43(DE3) facilitated by two fusion partners: Mistic and TarCF, a C-terminal fragment of the bacterial chemosensory transducer Tar at the C-terminal of the CB2 open reading frame region. | Mistic and TarCF as fusion protein partners for functional expression of the cannabinoid receptor 2 in Escherichia coli.
Chowdhury A, Feng R, Tong Q, Zhang Y, Xie XQ., Free PMC Article | 09/29/2012 |
| characterization of structural features of carboxyl-terminal 40 residues of Tar; identifed carboxyl-terminal linker between receptor body and the pentapeptide is an unstructured, disordered segment that can serve as a flexible arm and enzyme tether | Direct evidence that the carboxyl-terminal sequence of a bacterial chemoreceptor is an unstructured linker and enzyme tether.
Bartelli NL, Hazelbauer GL., Free PMC Article | 04/7/2012 |
| Ligand specificity is determined by differentially arranged common ligand-binding residues in bacterial amino acid chemoreceptors Tsr and Tar | Ligand specificity determined by differentially arranged common ligand-binding residues in bacterial amino acid chemoreceptors Tsr and Tar.
Tajima H, Imada K, Sakuma M, Hattori F, Nara T, Kamo N, Homma M, Kawagishi I., Free PMC Article | 02/25/2012 |
| Tar molecules with the cytoplasmic methylation and kinase control domains of Tsr still sensed phenol as an attractant. | Phenol sensing by Escherichia coli chemoreceptors: a nonclassical mechanism.
Pham HT, Parkinson JS., Free PMC Article | 01/7/2012 |
| The cytoplasmic domains of Tar and Tsr receptors are close to each other near the trimer contact region at the cytoplasmic tip. | Cross-linking evidence for motional constraints within chemoreceptor trimers of dimers.
Massazza DA, Parkinson JS, Studdert CA., Free PMC Article | 04/16/2011 |
| A mutational analysis of Tar residues 505-548 reveals that the more of this region is deleted, the less sensitive Tar is to inhibition by aspartate. | The region preceding the C-terminal NWETF pentapeptide modulates baseline activity and aspartate inhibition of Escherichia coli Tar.
Lai RZ, Bormans AF, Draheim RR, Wright GA, Manson MD. | 01/21/2010 |
| The pentapeptide linker in Tar is important for chemotaxis because of its role in adaptational modification. | The carboxyl-terminal linker is important for chemoreceptor function.
Li M, Hazelbauer GL. | 01/21/2010 |
| polar localization of Tar fused to green fluorescent protein is influenced by its own amidation (methylation) state and the expression of another chemoreceptor (Tsr) | Stabilization of polar localization of a chemoreceptor via its covalent modifications and its communication with a different chemoreceptor.
Shiomi D, Banno S, Homma M, Kawagishi I., Free PMC Article | 01/21/2010 |
| The organization of these receptor/signaling complexes is determined by conserved interactions between the constituent chemotaxis proteins and may represent the active form in vivo. | Self-assembly of receptor/signaling complexes in bacterial chemotaxis.
Wolanin PM, Baker MD, Francis NR, Thomas DR, DeRosier DJ, Stock JB., Free PMC Article | 01/21/2010 |
| These results with Tar-EnvZ hybrid receptor mutants suggest that intersubunit interactions in the HAMP linker normally mediate signal transduction through both subunits in a sensor dimer. | The HAMP linker in histidine kinase dimeric receptors is critical for symmetric transmembrane signal transduction.
Zhu Y, Inouye M. | 01/21/2010 |
| Data show that Tar can be readily mutated to respond to new chemical signals, but that the overall change in specificity depends on the target compound. | Changing the specificity of a bacterial chemoreceptor.
Derr P, Boder E, Goulian M. | 01/21/2010 |
| The tryptophan residues flanking the second transmembrane helix of Tar, especially Trp-209, are crucial in setting the baseline activity and ligand sensitivity of this chemoreceptor. | Tryptophan residues flanking the second transmembrane helix (TM2) set the signaling state of the Tar chemoreceptor.
Draheim RR, Bormans AF, Lai RZ, Manson MD. | 01/21/2010 |
| assisted adaptational modification (methylation, demthylation and deamidation) in vitro of Trg chemorecptor | Adaptational assistance in clusters of bacterial chemoreceptors.
Li M, Hazelbauer GL. | 01/21/2010 |
| mutations in the protein motifs of Tar significantly reduced the ability of the transmembrane domains to dimerize | Arginine mutations within a transmembrane domain of Tar, an Escherichia coli aspartate receptor, can drive homodimer dissociation and heterodimer association in vivo.
Sal-Man N, Shai Y., Free PMC Article | 01/21/2010 |
| The fundamental mechanism of transmembrane signaling is conserved between homodimeric sensor kinases (with NarX) and chemoreceptors. | Mutationally altered signal output in the Nart (NarX-Tar) hybrid chemoreceptor.
Ward SM, Bormans AF, Manson MD., Free PMC Article | 01/21/2010 |
| Data suggest that Tar()-Tsr* suppression most likely occurs through compensatory changes in the conformation or dynamics of a mixed receptor signaling complex, presumably based on trimer-of-dimer interactions. | Conformational suppression of inter-receptor signaling defects.
Ames P, Parkinson JS., Free PMC Article | 01/21/2010 |