| Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters. | Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
Saleh MM, Jeronimo C, Robert F, Zentner GE., Free PMC Article | 11/22/2021 |
| A framework for understanding how the efficiency of Mot1's catalytic activity is tuned to establish a dynamic pool of TBP without interfering with stable and functional TBP-containing complexes. | Conformational changes and catalytic inefficiency associated with Mot1-mediated TBP-DNA dissociation.
Heiss G, Ploetz E, Voith von Voithenberg L, Viswanathan R, Glaser S, Schluesche P, Madhira S, Meisterernst M, Auble DT, Lamb DC., Free PMC Article | 11/16/2019 |
| These results indicate that Rpa49, Rrn3 and Mot1 directly or indirectly influence biogenesis | Identification of factors that promote biogenesis of tRNA(CGA)(Ser).
Xu F, Zhou Y, Byström AS, Johansson MJO., Free PMC Article | 01/12/2019 |
| Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 co-localize on chromatin and coordinately suppress pervasive transcription in S. cerevisiae. | Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
Xue Y, Pradhan SK, Sun F, Chronis C, Tran N, Su T, Van C, Vashisht A, Wohlschlegel J, Peterson CL, Timmers HTM, Kurdistani SK, Carey MF., Free PMC Article | 09/30/2017 |
| these results suggest that TBP-DNA affinity as well as other aspects of promoter sequence influence Mot1 function in vivo. | Molecular Mechanism of Mot1, a TATA-binding Protein (TBP)-DNA Dissociating Enzyme.
Viswanathan R, True JD, Auble DT., Free PMC Article | 05/13/2017 |
| These results suggest that the large scale overlap in Mot1 and Spt16 function arises from a combination of both their unique and shared functions in transcription complex assembly and chromatin structure regulation. | The Modifier of Transcription 1 (Mot1) ATPase and Spt16 Histone Chaperone Co-regulate Transcription through Preinitiation Complex Assembly and Nucleosome Organization.
True JD, Muldoon JJ, Carver MN, Poorey K, Shetty SJ, Bekiranov S, Auble DT., Free PMC Article | 05/7/2017 |
| The results stress the importance of the cooperation of Mot1p/NC2 with remodelers in maintaining a repressive environment for intragenic transcription. | Suppression of intragenic transcription requires the MOT1 and NC2 regulators of TATA-binding protein.
Koster MJ, Yildirim AD, Weil PA, Holstege FC, Timmers HT., Free PMC Article | 07/12/2014 |
| The action of Mot1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites. | Mot1 redistributes TBP from TATA-containing to TATA-less promoters.
Zentner GE, Henikoff S., Free PMC Article | 02/1/2014 |
| In the absence of ATP, ternary complex stability arises from both Mot1-DNA and Mot1-TBP interactions. | Two-step mechanism for modifier of transcription 1 (Mot1) enzyme-catalyzed displacement of TATA-binding protein (TBP) from DNA.
Moyle-Heyrman G, Viswanathan R, Widom J, Auble DT., Free PMC Article | 06/16/2012 |
| findings suggest that the four conserved regions of Mot1 are essential for viability and required for proper regulation of most Mot1-regulated genes; loss of the ATPase domain, however, imparts some unexpected regulation on certain genes | Genome-wide transcriptional dependence on conserved regions of Mot1.
Venters BJ, Irvin JD, Gramlich P, Pugh BF., Free PMC Article | 07/23/2011 |
| Mot1 plays a broad role in establishing the precision and efficiency of RNA synthesis. | RNA synthesis precision is regulated by preinitiation complex turnover.
Poorey K, Sprouse RO, Wells MN, Viswanathan R, Bekiranov S, Auble DT., Free PMC Article | 03/19/2011 |
| MOT1 might have a role in vivo as a transcriptional activator in the small ubiquitin-related modifier (SUMO) protein pathway. | Genetic analysis connects SLX5 and SLX8 to the SUMO pathway in Saccharomyces cerevisiae.
Wang Z, Jones GM, Prelich G., Free PMC Article | 01/21/2010 |
| Mot1-mediated dissociation of TBP (or TBP-containing complexes) from chromatin can explain the Mot1 activation mechanism at some promoters. | TATA-binding protein variants that bypass the requirement for Mot1 in vivo.
Sprouse RO, Wells MN, Auble DT., Free PMC Article | 01/21/2010 |
| These results therefore demonstrate that Mot1 is an in vivo SUMO-targeted ubiquitin ligase target in yeast and suggest a role for SUMO-targeted degradation in protein quality control. | Quality control of a transcriptional regulator by SUMO-targeted degradation.
Wang Z, Prelich G., Free PMC Article | 01/21/2010 |
| Mot1p interacts with a range of transcriptional co-regulators as well as proteins involved in chromatin remodeling. | A proteomics analysis of yeast Mot1p protein-protein associations: insights into mechanism.
Arnett DR, Jennings JL, Tabb DL, Link AJ, Weil PA., Free PMC Article | 01/21/2010 |
| Mot1-mediated activation of URA1 transcription involves at least two steps, one of which is the removal of TBP bound to the promoter in the opposite orientation required for URA1 transcription. | Function and structural organization of Mot1 bound to a natural target promoter.
Sprouse RO, Shcherbakova I, Cheng H, Jamison E, Brenowitz M, Auble DT., Free PMC Article | 01/21/2010 |
| promoter dissociation of TBP and NC2 is highly dynamic, which is dependent on Mot1p function. Based on these results, we propose that NC2 and Mot1p cooperate to dynamically restrict TBP activity on transcribed promoters. | Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome.
van Werven FJ, van Bakel H, van Teeffelen HA, Altelaar AF, Koerkamp MG, Heck AJ, Holstege FC, Timmers HT., Free PMC Article | 01/21/2010 |
| These results support a model in which Mot1 affects the rate and efficiency of rRNA synthesis by both direct and indirect mechanisms, with resulting effects on transcription activation and the coupling of rRNA synthesis to processing. | Regulation of rRNA synthesis by TATA-binding protein-associated factor Mot1.
Dasgupta A, Sprouse RO, French S, Aprikian P, Hontz R, Juedes SA, Smith JS, Beyer AL, Auble DT., Free PMC Article | 01/21/2010 |
| Spt3 and Mot1 cooperate in nucleosome remodeling independently of TATA-binding protein recruitment. | Spt3 and Mot1 cooperate in nucleosome remodeling independently of TBP recruitment.
Topalidou I, Papamichos-Chronakis M, Thireos G, Tzamarias D., Free PMC Article | 01/21/2010 |
| Combining either a nhp6ab or a gcn5 mutation with mot1, ccr4, not4, or not5 mutations results in lethality. | Genetic interactions between Nhp6 and Gcn5 with Mot1 and the Ccr4-Not complex that regulate binding of TATA-binding protein in Saccharomyces cerevisiae.
Biswas D, Yu Y, Mitra D, Stillman DJ., Free PMC Article | 01/21/2010 |
| A novel ATP-independent TBPc displacement activity for Mot1 is reported | Snf2/Swi2-related ATPase Mot1 drives displacement of TATA-binding protein by gripping DNA.
Sprouse RO, Brenowitz M, Auble DT., Free PMC Article | 01/21/2010 |
| Data demonstrate functional links between SAGA, Mot1p, and TFIID in HXT gene regulation. | Differential requirement of SAGA subunits for Mot1p and Taf1p recruitment in gene activation.
van Oevelen CJ, van Teeffelen HA, Timmers HT., Free PMC Article | 01/21/2010 |