| Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes. | Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.
Maksiutenko EM, Barbitoff YA, Matveenko AG, Moskalenko SE, Zhouravleva GA., Free PMC Article | 02/12/2022 |
| Transcriptome-wide investigation of stop codon readthrough in Saccharomyces cerevisiae. | Transcriptome-wide investigation of stop codon readthrough in Saccharomyces cerevisiae.
Mangkalaphiban K, He F, Ganesan R, Wu C, Baker R, Jacobson A., Free PMC Article | 08/28/2021 |
| Mechanisms that ensure speed and fidelity in eukaryotic translation termination. | Mechanisms that ensure speed and fidelity in eukaryotic translation termination.
Lawson MR, Lessen LN, Wang J, Prabhakar A, Corsepius NC, Green R, Puglisi JD., Free PMC Article | 08/28/2021 |
| The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis. | The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis.
Lacoux C, Wacheul L, Saraf K, Pythoud N, Huvelle E, Figaro S, Graille M, Carapito C, Lafontaine DLJ, Heurgué-Hamard V., Free PMC Article | 01/2/2021 |
| Defects in the Dbp5-guided eRF1 delivery lead to premature contact and premature dissociation of eRF1 and eRF3 from the ribosome and to subsequent stop codon readthrough. Thus, the stepwise Dbp5-controlled termination complex assembly is essential for regular translation termination events | Translation termination depends on the sequential ribosomal entry of eRF1 and eRF3.
Beißel C, Neumann B, Uhse S, Hampe I, Karki P, Krebber H., Free PMC Article | 11/30/2019 |
| The study used analytical ultracentrifugation with fluorescent detection system to identify the protein complexome of eRF1 in Saccharomyces cerevisiae. In addition to eRF1 presence in translating polysomes, the study found that eRF1 is associated with five other macromolecular complexes: 77S, 57S, 39S, 28S, and 20S in size. | Defining the protein complexome of translation termination factor eRF1: Identification of four novel eRF1-containing complexes that range from 20S to 57S in size.
Denis CL, Richardson R, Park S, Zhang C, Xi W, Laue TM, Wang X., Free PMC Article | 06/29/2019 |
| We demonstrated that interaction of [SWI+] and [PIN+] causes inactivation of SUP45 gene that leads to nonsense suppression. Our data show that prion interactions may cause heritable traits in Saccharomyces cerevisiae. | Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae.
Nizhnikov AA, Ryzhova TA, Volkov KV, Zadorsky SP, Sopova JV, Inge-Vechtomov SG, Galkin AP., Free PMC Article | 05/13/2017 |
| In this work, the authors show that nonsense and missense mutations in the SUP45, but not the SUP35, gene abolish diploid pseudohyphal and haploid invasive growth. | The translation termination factor eRF1 (Sup45p) of Saccharomyces cerevisiae is required for pseudohyphal growth and invasion.
Petrova A, Kiktev D, Askinazi O, Chabelskaya S, Moskalenko S, Zemlyanko O, Zhouravleva G. | 02/27/2016 |
| Analysis of the L123 systematic mutants of eRF1 suggested that this amino acid functions in stop codon discrimination in a manner coupled with eRF3 binding, and distinctive from previously reported adjacent residues. | Genetic analysis of L123 of the tRNA-mimicking eukaryote release factor eRF1, an amino acid residue critical for discrimination of stop codons.
Saito K, Ito K., Free PMC Article | 08/1/2015 |
| We characterized a region of the eRF1 N-terminal domain, the P1 pocket, that we had previously shown to be involved in termination efficiency. We identified two residues, arginine 65 and lysine 109, as critical for recognition of the three stop codons. | New insights into stop codon recognition by eRF1.
Blanchet S, Rowe M, Von der Haar T, Fabret C, Demais S, Howard MJ, Namy O., Free PMC Article | 06/27/2015 |
| Prion-like determinant [NSI+] decreases the relative amounts of SUP45 mRNA that causes a decrease in the amounts of Sup45 protein. | [Prion-like determinant [NSI+] decreases expression of the SUP45 gene in Saccharomyces cerevisiae].
Kondrashkina AM, Antonets KS, Galkin AP, Nizhnikov AA. | 06/6/2015 |
| HLJ1 and TEF2 genes are affecting Sup35 protein prionization and/or the efficiency of translation termination. | [The identification of Saccharomyces cerevisiae genes leading to synthetic lethality of prion [PSI+] with Sup45 mutations].
Matveenko AG, Zemlianko OM, Zhuravleva GA. | 03/15/2014 |
| Data indicate that SUP35 ORF (eRF3) affects binding between SUP45 ORF (eRF1) and the ribosome, either prior to or subsequent to peptide release. | Eukaryotic release factor 3 is required for multiple turnovers of peptide release catalysis by eukaryotic release factor 1.
Eyler DE, Wehner KA, Green R., Free PMC Article | 12/14/2013 |
| A deterministic mathematical model of the eRF1 (SUP45) feedback loop was developed using a staged increase in model complexity. | Regulation of release factor expression using a translational negative feedback loop: a systems analysis.
Betney R, de Silva E, Mertens C, Knox Y, Krishnan J, Stansfield I., Free PMC Article | 02/2/2013 |
| The results indicated that changes near cavities two and three frequently mediated significant effects on stop codon selectivity by eRF1 (SUP45). Changes in the YCF motif correlated most consistently with variant code stop codon selectivity. | Identification of eRF1 residues that play critical and complementary roles in stop codon recognition.
Conard SE, Buckley J, Dang M, Bedwell GJ, Carter RL, Khass M, Bedwell DM., Free PMC Article | 07/28/2012 |
| Deletion of each UPF gene leads to allosuppresson of ade1-14 mutation without changing eRF1 amount | [The influence of UPF genes on the severity of SUP45 mutations].
Zhuravleva GA, Gryzina VA. | 06/23/2012 |
| SUP45 gene modulates nonsense suppression in S. cerevisiae | [NSI+] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes.
Nizhnikov AA, Magomedova ZM, Rubel AA, Kondrashkina AM, Inge-Vechtomov SG, Galkin AP. | 06/2/2012 |
| The role of translation termination factor eRF1 in the regulation of pseudohyphal growth in Saccharomyces cerevisiae cells. | The role of translation termination factor eRF1 in the regulation of pseudohyphal growth in Saccharomyces cerevisiae cells.
Zhouravleva GA, Petrova AV. | 12/4/2010 |
| Results indicate that point mutants of Saccharomyces cerevisiae eRF1 display significant variability in their stop codon read-through phenotypes. | Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast.
Merritt GH, Naemi WR, Mugnier P, Webb HM, Tuite MF, von der Haar T., Free PMC Article | 10/23/2010 |
| although the Fe-S cluster is not required for the interaction of Rli1 with eRF1 or its other interacting partner, Hcr1, from the initiation complex eIF3, it is required for its activity in translation termination | The iron-sulphur protein RNase L inhibitor functions in translation termination.
Khoshnevis S, Gross T, Rotte C, Baierlein C, Ficner R, Krebber H., Free PMC Article | 08/9/2010 |
| Overexpression of genes encoding tRNA(Tyr) AND tRNA(Gln) improves viability of nonsense mutants in SUP45 gene in yeast Saccharomyces cerevisiae | [Overexpression of genes encoding tRNA(Tyr) AND tRNA(Gln) improves viability of nonsense mutants in SUP45 gene in yeast Saccharomyces cerevisiae].
Murina OA, Moskalenko SE, Zhuravleva GA. | 08/2/2010 |
| This suggests [PSI(+)] toxicity caused by excess Sup35p verses Sup35NMp is, respectively, through sequestration/inactivation of Sup45p verses Sup35p. | Sequestration of essential proteins causes prion associated toxicity in yeast.
Vishveshwara N, Bradley ME, Liebman SW., Free PMC Article | 01/21/2010 |
| TEF4 and TEF3/CAM1) and alpha (TEF5/EFB1) subunits of the translation elongation factor eEF1B act as dosage suppressors of a synthetic growth defect caused by some mutations in the SUP45 and SUP35 genes encoding eRF1 and eRF3, respectively. | Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeast.
Valouev IA, Fominov GV, Sokolova EE, Smirnov VN, Ter-Avanesyan MD., Free PMC Article | 01/21/2010 |
| compared readthrough efficiency of the natural termination codon of SUP45 gene, spontaneous sup45-n (nonsense) mutations, nonsense mutations obtained by site-directed mutagenesis (76Q --> TAA, 242R --> TGA, 317L --> TAG) | The paradox of viable sup45 STOP mutations: a necessary equilibrium between translational readthrough, activity and stability of the protein.
Kiktev D, Moskalenko S, Murina O, Baudin-Baillieu A, Rousset JP, Zhouravleva G. | 01/21/2010 |
| mutagenesis of SUP45 led to the identification of two new pockets in domain 1 (P1 and P2) involved in the regulation of eRF1 activity. | Molecular dissection of translation termination mechanism identifies two new critical regions in eRF1.
Hatin I, Fabret C, Rousset JP, Namy O., Free PMC Article | 01/21/2010 |