| TRAPPIII requires Drs2 binding to transport Atg9 vesicles at cold temperatures. | TRAPPIII requires Drs2 binding to transport Atg9 vesicles at cold temperatures.
Puig-Tintó M, Pazos I, Betancur L, Hernández AC, Gallego O., Free PMC Article | 10/16/2023 |
| The P4-ATPase Drs2 interacts with and stabilizes the multisubunit tethering complex TRAPPIII in yeast. | The P4-ATPase Drs2 interacts with and stabilizes the multisubunit tethering complex TRAPPIII in yeast.
Pazos I, Puig-Tintó M, Betancur L, Cordero J, Jiménez-Menéndez N, Abella M, Hernández AC, Duran AG, Adachi-Fernández E, Belmonte-Mateos C, Sabido-Bozo S, Tosi S, Nezu A, Oliva B, Colombelli J, Graham TR, Yoshimori T, Muñiz M, Hamasaki M, Gallego O., Free PMC Article | 05/8/2023 |
| The Drs2 PS flippase activity is required to support neo1Delta any1Delta viability. | The PQ-loop protein Any1 segregates Drs2 and Neo1 functions required for viability and plasma membrane phospholipid asymmetry.
Takar M, Huang Y, Graham TR., Free PMC Article | 08/15/2020 |
| cryo-electron microscopy structure of the P4-ATPase Drs2p-Cdc50p; three structures are presented that represent the complex in an autoinhibited, an intermediate and a fully activated state | Structure and autoregulation of a P4-ATPase lipid flippase.
Timcenko M, Lyons JA, Januliene D, Ulstrup JJ, Dieudonné T, Montigny C, Ash MR, Karlsen JL, Boesen T, Kühlbrandt W, Lenoir G, Moeller A, Nissen P. | 10/12/2019 |
| activation of the Drs2p-Cdc50p flippase follows a multistep mechanism, with preliminary release of a number of constraints, possibly through the binding of regulatory proteins in the trans-Golgi network, followed by full activation by PI4P | High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions.
Azouaoui H, Montigny C, Dieudonné T, Champeil P, Jacquot A, Vázquez-Ibar JL, Le Maréchal P, Ulstrup J, Ash MR, Lyons JA, Nissen P, Lenoir G., Free PMC Article | 07/15/2017 |
| Deletion of KES1 suppresses plasma membrane-missorting defects and the accumulation of intracellular ergosterol in drs2 mutants. | Phosphatidylserine translocation at the yeast trans-Golgi network regulates protein sorting into exocytic vesicles.
Hankins HM, Sere YY, Diab NS, Menon AK, Graham TR., Free PMC Article | 10/1/2016 |
| we describe the overexpression of a yeast lipid flippase complex, namely the P4-ATPase Drs2p and its associated subunit Cdc50p, in a coordinated manner. | Coordinated Overexpression in Yeast of a P4-ATPase and Its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex.
Azouaoui H, Montigny C, Jacquot A, Barry R, Champeil P, Lenoir G. | 09/24/2016 |
| Imh1p and Drs2p modulate the activity of Gcs1p by timing its interaction with Arl1p, hence providing feedback regulation of Arl1p activity. | Mechanism of action of the flippase Drs2p in modulating GTP hydrolysis of Arl1p.
Hsu JW, Chen ZJ, Liu YW, Lee FJ. | 01/31/2015 |
| Auto-inhibition of Drs2p, a yeast phospholipid flippase, by its carboxyl-terminal tail. | Auto-inhibition of Drs2p, a yeast phospholipid flippase, by its carboxyl-terminal tail.
Zhou X, Sebastian TT, Graham TR., Free PMC Article | 01/4/2014 |
| results demonstrate that activated Arl1p can promote the spatial modulation of membrane organization at the trans-Golgi network through interacting with the effectors Gea2p and Drs2p | Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p.
Tsai PC, Hsu JW, Liu YW, Chen KY, Lee FJ., Free PMC Article | 04/27/2013 |
| Phosphatidylinositol-4-phosphate tightly controls Drs2p-catalyzed lipid transport and establish the functional relevance of the Drs2p.Cdc50p complex. | Phosphatidylserine stimulation of Drs2p·Cdc50p lipid translocase dephosphorylation is controlled by phosphatidylinositol-4-phosphate.
Jacquot A, Montigny C, Hennrich H, Barry R, le Maire M, Jaxel C, Holthuis J, Champeil P, Lenoir G., Free PMC Article | 06/23/2012 |
| Drs2p can catalyze phospholipid translocation directly through purification and reconstitution of this P4-ATPase into proteoliposomes. | Reconstitution of phospholipid translocase activity with purified Drs2p, a type-IV P-type ATPase from budding yeast.
Zhou X, Graham TR., Free PMC Article | 01/21/2010 |
| Drs2p, a type IV P-type ATPase required for phospholipid translocase (flippase) activity and transport vesicle budding from the trans-Golgi network, is an effector of phosphatidylinositol-4-phosphate. | Regulation of a Golgi flippase by phosphoinositides and an ArfGEF.
Natarajan P, Liu K, Patil DV, Sciorra VA, Jackson CL, Graham TR., Free PMC Article | 01/21/2010 |
| the affinity of yeast P(4)-ATPase Drs2p for its Cdc50-binding partner fluctuates during the transport cycle, with the strongest interaction occurring at a point where the enzyme is loaded with phospholipid ligand | Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter Drs2p.
Lenoir G, Williamson P, Puts CF, Holthuis JC., Free PMC Article | 01/21/2010 |
| Potential phospholipid translocases in the Drs2/Dnf family (type IV P-type ATPases [P4-ATPases]) are downstream targets of Kes1p repression. | Control of protein and sterol trafficking by antagonistic activities of a type IV P-type ATPase and oxysterol binding protein homologue.
Muthusamy BP, Raychaudhuri S, Natarajan P, Abe F, Liu K, Prinz WA, Graham TR., Free PMC Article | 01/21/2010 |
| Drs2p acts independently of coat recruitment to facilitate AP-1/clathrin-coated vesicle budding from the TGN | P4-ATPase requirement for AP-1/clathrin function in protein transport from the trans-Golgi network and early endosomes.
Liu K, Surendhran K, Nothwehr SF, Graham TR., Free PMC Article | 01/21/2010 |
| Drs2p is an essential endocytic cargo in cells compromised for Ub-dependent endocytosis. | Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway.
Liu K, Hua Z, Nepute JA, Graham TR., Free PMC Article | 01/21/2010 |
| These results suggest that Cdc50p-Drs2p plays an important role in the Arf1p/Gcs1-mediated formation of clathrin-coated vesicles for the retrieval pathway from early endosomes to the trans-Golgi network. | The functional relationship between the Cdc50p-Drs2p putative aminophospholipid translocase and the Arf GAP Gcs1p in vesicle formation in the retrieval pathway from yeast early endosomes to the TGN.
Sakane H, Yamamoto T, Tanaka K. | 01/21/2010 |
| Cdc50p, a membrane protein in the endosomal/trans-Golgi network compartments, is a noncatalytic subunit of Drs2p, which is implicated in translocation of phospholipids across lipid bilayers. | Defects in structural integrity of ergosterol and the Cdc50p-Drs2p putative phospholipid translocase cause accumulation of endocytic membranes, onto which actin patches are assembled in yeast.
Kishimoto T, Yamamoto T, Tanaka K., Free PMC Article | 01/21/2010 |
| Drs2 or cdc50 null mutatations cause a loss of plasma membrane PS asymmetry. Loss of plasma membrane asymmetry is a secondary consequence of disrupting protein trafficking. | Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane.
Chen S, Wang J, Muthusamy BP, Liu K, Zare S, Andersen RJ, Graham TR. | 01/21/2010 |