| PiggyBac transposon system proved to be a fast and efficient method to generate cells stably expressing SolCD39, producing high amounts of the enzyme from a limited number of cells and with high hydrolytic activity. | Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level.
Beckenkamp LR, Iser IC, Onzi GR, Fontoura DMSD, Bertoni APS, Sévigny J, Lenz G, Wink MR. | 02/29/2020 |
| Studied effect of exercise on memory and cerebral cortex physiology of NTPDase-1 (CD39), 5' nucleotidase ecto (CD73), and adenosine A2a receptor (A2ar). | Physical exercise prevents memory impairment in an animal model of hypertension through modulation of CD39 and CD73 activities and A2A receptor expression.
Cardoso AM, Manfredi LH, Zanini D, Bagatini MD, Gutierres JM, Carvalho F, Tremblay A, Belló-Klein A, Rubin MA, Morsch VM, Sévigny J, Schetinger MRC. | 01/25/2020 |
| E-NTPDase activity in hepatic lymphocytes differs from its activity profile in peripheral lymphocytes. | Characterization of E-NTPDase (EC 3.6.1.5) activity in hepatic lymphocytes: A different activity profile from peripheral lymphocytes.
Doleski PH, Adefegha SA, Cabral FL, Leal DB. | 03/18/2017 |
| E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis. | E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans.
de Azevedo MI, Ferreiro L, Da Silva AS, Tonin AA, Ruchel JB, Rezer JF, França RT, Zimmermann CE, Leal DB, Duarte MM, Lopes ST, Flores MM, Fighera R, Santurio JM. | 06/20/2015 |
| two ENTPD isoforms, ENTPD-1 and -2, are expressed in adult rat ventricular CFs | Hydrolysis of extracellular ATP by ectonucleoside triphosphate diphosphohydrolase (ENTPD) establishes the set point for fibrotic activity of cardiac fibroblasts.
Lu D, Insel PA., Free PMC Article | 09/14/2013 |
| NTPDase activity is increased in lymphocytes during experimental sepsis | Increased NTPDase activity in lymphocytes during experimental sepsis.
Bertoncheli Cde M, Zimmermann CE, Jaques JA, Leal CA, Ruchel JB, Rocha BC, Pinheiro Kde V, Souza Vdo C, Stainki DR, Luz SC, Schetinger MR, Leal DB., Free PMC Article | 11/24/2012 |
| These data suggest that NTPDase activity is altered on the surface of lymphocytes of rats infected with T. evansi at different time-points of infection. | NTPDase activity in lymphocytes of rats infected by Trypanosoma evansi.
Oliveira CB, da Silva AS, Souza VC, Costa MM, Jaques JA, Leal DB, Lopes ST, Monteiro SG. | 06/9/2012 |
| An interdomain rotation angle of up to 7.4 degrees affects the active site cleft between the two lobes of the protein. | Crystallographic evidence for a domain motion in rat nucleoside triphosphate diphosphohydrolase (NTPDase) 1.
Zebisch M, Krauss M, Schäfer P, Sträter N. | 03/3/2012 |
| NTPDase activity can act as purinergic and cholinergic markers in lymphocytes | In vitro and in vivo interactions of aluminum on NTPDase and AChE activities in lymphocytes of rats.
Kaizer RR, Gutierres JM, Schmatz R, Spanevello RM, Morsch VM, Schetinger MR, Rocha JB. | 11/27/2010 |
| A significant increase in NTPDase activity was observed in lymphocytes (about 30% in ATP hydrolysis and 80% in ADP hydrolysis, at p<0.05 and p<0.01, respectively). | Enzymes that hydrolyze adenine nucleotides in lymphocytes and platelets of immunosuppressed rats.
Saucedo EM, Pereira RS, Barbosa GM, Battisti V, Leal CA, Fleck J, Santos RC, Morsch VM, Schetinger MR, Leal DB. | 09/20/2010 |
| Data show that ectonucleotidase and 5' nucleotidase activities in synaptosomes and platelets were altered after a demyelination and that vitamin E modulated adenine nucleotide hydrolysis and oxidative stress parameters in this experimental condition. | Effect of vitamin E on ectonucleotidase activities in synaptosomes and platelets and parameters of oxidative stress in rats experimentally demyelinated.
Spanevello R, Mazzanti CM, Schmatz R, Bagatini M, Stefanello N, Correa M, Kaizer R, Maldonado P, Mazzanti A, Graça DL, Martins TB, Danesi C, Morsch VM, Schetinger MR. | 01/21/2010 |
| soluble apyrase as well as the glomerular ENTPD1 are sensitive to oxidant stress, which may explain their downregulation in the ischemic condition in vivo | Histochemical detection of ischemia-like alterations induced in kidney tissue in vitro--different sensitivity to oxidant stress of glomerular ENTPD1 versus E5NT.
Vlaar AP, van Son WJ, Bakker WW. | 01/21/2010 |
| The solubilized enzyme E-NTPDase1 shows high- and low-affinity sites for the substrate in contrast with a single substrate site for the membrane-bound enzyme. In addition, the site-site interactions after solubilization resulted in a less active enzyme. | Removal from the membrane affects the interaction of rat osseous plate ecto-nucleosidetriphosphate diphosphohydrolase-1 with substrates and ions.
Garçon DP, Masui DC, Furriel RP, Leone FA. | 01/21/2010 |
| Our results suggest that selenium compounds, as well as the well-known antioxidant trolox, can avoid the increase on glutamate-induced stimulation of ecto-nucleotidase activities probably due to their antioxidant properties. | Selenium compounds counteract the stimulation of ecto-nucleotidase activities in rat cultured cerebellar granule cells: putative correlation with neuroprotective effects.
Ghisleni G, Porciúncula LO, Mioranzza S, Boeck CR, Rocha JB, Souza DO. | 01/21/2010 |
| Results describe the expression, purification and crystallization of the ecto-enzymatic domain of rat CD39 (NTPDase 1), an ecto-nucleoside triphosphate diphosphohydrolase. | Expression, purification and crystallization of the ecto-enzymatic domain of rat E-NTPDase1 CD39.
Zhong X, Buddha M, Guidotti G, Kriz R, Somers W, Mosyak L., Free PMC Article | 01/21/2010 |
| results suggest that alterations caused by guanidinoacetate in the activities of NTPDase, 5'-nucleotidase and acetylcholinesterase may contribute to the understanding of the neurological dysfunction of Guanidinoacetate methyltransferase-deficient patients | Effects in vitro of guanidinoacetate on adenine nucleotide hydrolysis and acetylcholinesterase activity in tissues from adult rats.
Spanevello RM, de Souza Wyse AT, Mazzanti CM, Schmatz R, Stefanello N, Gonçalves JF, Bagatini M, Battisti V, Morsch VM, Schetinger MR. | 01/21/2010 |
| Al increases NTPDase and 5'-nucleotidase activities, in synaptosomal fractions and platelets. Thus, we suggest that platelets could be sensitive peripheral markers of Al toxicity of the central nervous system. | The effect of aluminium on NTPDase and 5'-nucleotidase activities from rat synaptosomes and platelets.
Kaizer RR, Maldonado PA, Spanevello RM, Corrêa MC, Gonçalves JF, Becker LV, Morsch VM, Schetinger MR. | 01/21/2010 |
| Broad overlapping distribution in neurons and glial cells reflects the functional importance of ectonucleotidase actions in the nervous system. | Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution.
Bjelobaba I, Stojiljkovic M, Pekovic S, Dacic S, Lavrnja I, Stojkov D, Rakic L, Nedeljkovic N. | 01/21/2010 |
| Results describe the effect of arginine administration on NTPDase and 5'-nucleotidase activities in rat hippocampal synaptosomes. | NTPDase and 5'-nucleotidase activities of synaptosomes from hippocampus of rats subjected to hyperargininemia.
Delwing D, Delwing D, Gonçalves MC, Sarkis JJ, Wyse AT. | 01/21/2010 |
| NTPDase-1 expression profile was studied in the Walker 256 tumor. | NTPDase and 5' ecto-nucleotidase expression profiles and the pattern of extracellular ATP metabolism in the Walker 256 tumor.
Buffon A, Wink MR, Ribeiro BV, Casali EA, Libermann TA, Zerbini LF, Robson SC, Sarkis JJ. | 01/21/2010 |
| Results suggest that up-regulation of NTPDase 1 after cortical stab injury was predominantly in microglial cells, whereas for ecto 5'-nucleotidase was predominantly in astrocytes. | Up-regulation of ectonucleotidase activity after cortical stab injury in rats.
Nedeljkovic N, Bjelobaba I, Subasic S, Lavrnja I, Pekovic S, Stojkov D, Vjestica A, Rakic L, Stojiljkovic M. | 01/21/2010 |
| it is proposed on the basis of published data that E-NTPase/E-NTPDase could play a role in the influx and efflux of Ca2+and Mg2+ in vivo | Regulatory role of E-NTPase/E-NTPDase in Ca2+/Mg2+ transport via gated channel.
Schreiber HM, Kannan S., Free PMC Article | 01/21/2010 |
| The participation of ecto-ATP diphosphohydrolase (CD39; ecto-NTPDase) and ecto-5'-nucleotidase (CD73) activities in the nucleotide hydrolysis by salivary gland cells from rats was evaluated. | Kinetic characterization of ATP diphosphohydrolase and 5'-nucleotidase activities in cells cultured from submandibular salivary glands of rats.
Henz SL, Ribeiro CG, Rosa A, Chiarelli RA, Casali EA, Sarkis JJ. | 01/21/2010 |
| The transmembrane domains of NTPDase-1 regulate its enzymatic activity via a set of highly dynamic intra- and intersubunit interactions. | Dynamic motions of CD39 transmembrane domains regulate and are regulated by the enzymatic active site.
Grinthal A, Guidotti G. | 01/21/2010 |
| results indicate that rat CD39 can be glycosylated at all seven sites when expressed in COS7 cells [CD39] | N-linked oligosaccharides affect the enzymatic activity of CD39: diverse interactions between seven N-linked glycosylation sites.
Wu JJ, Choi LE, Guidotti G., Free PMC Article | 01/21/2010 |