| The atomic structure of an NHEJ junction with a Pol x construct that mimics Pol mu in a reconstituted system explained the distinctive properties of Pol mu compared with TdT. The structure suggested a mechanism of base selection relying on Loop1 and taking instructions via the in trans templating base independently of the primer strand. | Structural evidence for an in trans base selection mechanism involving Loop1 in polymerase μ at an NHEJ double-strand break junction.
Loc'h J, Gerodimos CA, Rosario S, Tekpinar M, Lieber MR, Delarue M., Free PMC Article | 03/14/2020 |
| Findings demonstrate that Polmu is necessary for proper retinal development, and support that the generation of DNA double-strand breaks and their repair via the non-homologous end-joining pathway are genuine processes involved in neural development. | Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina.
Baleriola J, Álvarez-Lindo N, de la Villa P, Bernad A, Blanco L, Suárez T, de la Rosa EJ., Free PMC Article | 04/14/2018 |
| A role for PolMU in the prevention of sarcomagenesis on a murine P53-deficient background was identified. | Polμ deficiency induces moderate shortening of P53(-/-) mouse lifespan and modifies tumor spectrum.
Escudero B, Herrero D, Torres Y, Cañón S, Molina A, Carmona RM, Suela J, Blanco L, Samper E, Bernad A. | 08/19/2017 |
| Pol mu and Pol lambda play a key role in conferring on NHEJ the flexibility required for accurate and efficient repair | Essential role for polymerase specialization in cellular nonhomologous end joining.
Pryor JM, Waters CA, Aza A, Asagoshi K, Strom C, Mieczkowski PA, Blanco L, Ramsden DA., Free PMC Article | 04/30/2016 |
| Polmu deficiency activates transcriptional networks that reduce constitutive apoptosis, leading to enhanced liver repair at old age. | Polμ deficiency increases resistance to oxidative damage and delays liver aging.
Escudero B, Lucas D, Albo C, Dhup S, Bacher JW, Sánchez-Muñoz A, Fernández M, Rivera-Torres J, Carmona RM, Fuster E, Carreiro C, Bernad R, González MA, Andrés V, Blanco L, Roche E, Fabregat I, Samper E, Bernad A., Free PMC Article | 12/19/2015 |
| DNA polymerase mu has a role in decreased learning and brain long-term potentiation in aged mice | Increased learning and brain long-term potentiation in aged mice lacking DNA polymerase μ.
Lucas D, Delgado-García JM, Escudero B, Albo C, Aza A, Acín-Pérez R, Torres Y, Moreno P, Enríquez JA, Samper E, Blanco L, Fairén A, Bernad A, Gruart A., Free PMC Article | 08/31/2013 |
| These data point to a critical role for pol mu as a global repair player that increases the efficiency and the fidelity of non-homologous end-joining. | DNA polymerase μ is a global player in the repair of non-homologous end-joining substrates.
Chayot R, Montagne B, Ricchetti M. | 08/11/2012 |
| pronounced senescence in pololymerase mu deficient cells | Lack of DNA polymerase μ affects the kinetics of DNA double-strand break repair and impacts on cellular senescence.
Chayot R, Danckaert A, Montagne B, Ricchetti M. | 03/19/2011 |
| A series of molecular-dynamics simulations with and without the incoming nucleotide in various forms, including mutant systems, based on pol mu's crystal ternary structure, were analyzed. | Modeling DNA polymerase μ motions: subtle transitions before chemistry.
Li Y, Schlick T., Free PMC Article | 03/5/2011 |
| Polm is required for hematopoietic development with an role in maintaining genetic stability in hematopoietic and non-hematopoietic tissues. | Altered hematopoiesis in mice lacking DNA polymerase mu is due to inefficient double-strand break repair.
Lucas D, Escudero B, Ligos JM, Segovia JC, Estrada JC, Terrados G, Blanco L, Samper E, Bernad A., Free PMC Article | 01/21/2010 |
| Polmu participates in the repair of early-embryo, RAG-induced double-strand breaks and subsequently may contribute to preserve the genomic stability and cellular homeostasis of lymphohematopoietic precursors during development | A role for DNA polymerase mu in the emerging DJH rearrangements of the postgastrulation mouse embryo.
Gozalbo-López B, Andrade P, Terrados G, de Andrés B, Serrano N, Cortegano I, Palacios B, Bernad A, Blanco L, Marcos MA, Gaspar ML., Free PMC Article | 01/21/2010 |
| Pol mu promotes accuracy during Ig kappa recombination. | A gradient of template dependence defines distinct biological roles for family X polymerases in nonhomologous end joining.
Nick McElhinny SA, Havener JM, Garcia-Diaz M, Juárez R, Bebenek K, Kee BL, Blanco L, Kunkel TA, Ramsden DA. | 01/21/2010 |
| Pol mu is not required for normal Ig gene hypermutation. | Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation.
Bertocci B, De Smet A, Flatter E, Dahan A, Bories JC, Landreau C, Weill JC, Reynaud CA. | 01/21/2010 |
| The crystal structure of the polymerase domain of murine Pol mu bound to gapped DNA with a correct dNTP at the active site reveals substrate interactions with side chains in Pol mu that differ from other family X members. | Structural insight into the substrate specificity of DNA Polymerase mu.
Moon AF, Garcia-Diaz M, Bebenek K, Davis BJ, Zhong X, Ramsden DA, Kunkel TA, Pedersen LC. | 01/21/2010 |
| third hypervariable region assumes for each immunoglobulin chain, with pol lambda maintaining a large heavy chain junctional heterogeneity and pol mu ensuring a restricted light chain junctional variability | Nonoverlapping functions of DNA polymerases mu, lambda, and terminal deoxynucleotidyltransferase during immunoglobulin V(D)J recombination in vivo.
Bertocci B, De Smet A, Weill JC, Reynaud CA. | 01/21/2010 |