| Employ genome engineering to generate a functional optogenetic allele of the Notch ligand Delta (opto-Delta), which replaces both copies of the endogenous wild-type locus. Using clonal analysis, we show that optogenetic activation blocks Notch activation through cis-inhibition in signal-receiving cells. | Optogenetic inhibition of Delta reveals digital Notch signalling output during tissue differentiation.
Viswanathan R, Necakov A, Trylinski M, Harish RK, Krueger D, Esposito E, Schweisguth F, Neveu P, De Renzis S., Free PMC Article | 05/23/2020 |
| Here we show that, in intestinal stem cell lineages in adult Drosophila, in which the Delta-Notch-signalling-guided progenitor cell differentiation into enterocytes is the default mode, the specification of enteroendocrine cells (EEs) is initiated by transient Scute activation in a process driven by transcriptional self-stimulation combined with a negative feedback regulation between Scute and Notch targets | Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells.
Chen J, Xu N, Wang C, Huang P, Huang H, Jin Z, Yu Z, Cai T, Jiao R, Xi R. | 04/13/2019 |
| Delta-Notch dependent boundary formation in the Drosophila large intestine | Modeling and analysis of the Delta-Notch dependent boundary formation in the Drosophila large intestine.
Liu F, Sun D, Murakami R, Matsuno H., Free PMC Article | 06/23/2018 |
| The authors found that Dl requires ubi for its full function, but can also signal in two ubi-independent modes, one dependent and one independent of Neur. Their analysis suggests that one important role of Dl protein ubiquitination by Mib1 is their release from cis-inhibitory interactions with Notch, enabling them to trans-activate Notch on adjacent cells. | Ubiquitylation-independent activation of Notch signalling by Delta.
Berndt N, Seib E, Kim S, Troost T, Lyga M, Langenbach J, Haensch S, Kalodimou K, Delidakis C, Klein T., Free PMC Article | 06/23/2018 |
| reciprocal relationship between Notch signalling and cell cycle progression acts like a developmental clock, providing a delimited window of time during which cells decide their fate, ensuring efficient and orderly bristle patterning. | Coordinated control of Notch/Delta signalling and cell cycle progression drives lateral inhibition-mediated tissue patterning.
Hunter GL, Hadjivasiliou Z, Bonin H, He L, Perrimon N, Charras G, Baum B., Free PMC Article | 09/9/2017 |
| A new paramutation-like example at the Delta gene of Drosophila has been described. | A new paramutation-like example at the Delta gene of Drosophila.
Capovilla M, Robichon A, Rassoulzadegan M., Free PMC Article | 08/26/2017 |
| Binding of Notch to ligands of the Delta/DLL and Serrate/JAG families from neighboring cells leads to activation of the signaling pathway and is called trans-activation | Xylosylation of the Notch receptor preserves the balance between its activation by trans-Delta and inhibition by cis-ligands in Drosophila.
Lee TV, Pandey A, Jafar-Nejad H., Free PMC Article | 06/3/2017 |
| miR-124 plays neuroprotective roles in Alzheimer's disease Drosophila by targeting Delta in Notch signaling pathway. | The Role of miR-124 in Drosophila Alzheimer's Disease Model by Targeting Delta in Notch Signaling Pathway.
Kong Y, Wu J, Zhang D, Wan C, Yuan L. | 09/10/2016 |
| We further show that Notch in ORNs acts by both canonical cleavage-dependent and non-canonical cleavage-independent pathways. The Notch ligand Delta (Dl) in PNs switches the balance between the pathways. | Notch is required in adult Drosophila sensory neurons for morphological and functional plasticity of the olfactory circuit.
Kidd S, Struhl G, Lieber T., Free PMC Article | 04/9/2016 |
| Myoblast cytonemes mediate Wg signaling from the wing imaginal disc and Delta-Notch signaling to the air sac primordium. | Myoblast cytonemes mediate Wg signaling from the wing imaginal disc and Delta-Notch signaling to the air sac primordium.
Huang H, Kornberg TB., Free PMC Article | 01/16/2016 |
| The Drosophila Mid proten functions within the Notch-Delta signaling pathway to specify sensory organ precursor cell fates and regulates cell survival within the eye imaginal disc. | The Drosophila T-box transcription factor Midline functions within the Notch-Delta signaling pathway to specify sensory organ precursor cell fates and regulates cell survival within the eye imaginal disc.
Das S, Chen QB, Saucier JD, Drescher B, Zong Y, Morgan S, Forstall J, Meriwether A, Toranzo R, Leal SM., Free PMC Article | 06/14/2014 |
| lateral inhibition promotes the robustness of tip-cell selection in the tracheal development of Drosophila | Mathematical study of the role of Delta/Notch lateral inhibition during primary branching of Drosophila trachea development.
Koizumi Y, Iwasa Y, Hirashima T., Free PMC Article | 07/6/2013 |
| A critical step in the activation of Notch signaling is the endocytosis of the Notch ligands Delta and Serrate. Ligand endocytosis is regulated by one of two E3 ubiquitin ligases, Neuralized (Neur) or Mind bomb. | Functional analysis of the NHR2 domain indicates that oligomerization of Neuralized regulates ubiquitination and endocytosis of Delta during Notch signaling.
Liu S, Bonner JM, Chanet S, Commisso C, Skwarek LC, Schweisguth F, Boulianne GL., Free PMC Article | 02/16/2013 |
| The ubiquitylation of Delta by both Neuralized and Mindbomb1 was detected, with the two enzymes using distinct docking sites and having different acceptor lysine preferences. | Distinct intracellular motifs of Delta mediate its ubiquitylation and activation by Mindbomb1 and Neuralized.
Daskalaki A, Shalaby NA, Kux K, Tsoumpekos G, Tsibidis GD, Muskavitch MA, Delidakis C., Free PMC Article | 02/11/2012 |
| A complex regulatory process in which the microRNA pathway promotes Notch activation by repressing Delta-mediated inhibition of Notch in follicle cells. | The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells.
Poulton JS, Huang YC, Smith L, Sun J, Leake N, Schleede J, Stevens LM, Deng WM., Free PMC Article | 07/2/2011 |
| Notch activation by odorants depends on the Delta/Serrate/Lag2 ligand Delta in select olfactory receptor neurons. | DSL-Notch signaling in the Drosophila brain in response to olfactory stimulation.
Lieber T, Kidd S, Struhl G., Free PMC Article | 04/16/2011 |
| Differential Vegfr levels affect tip selection only in the presence of a functional Notch system by modulating the expression of the ligand Dll4. | Endothelial cells dynamically compete for the tip cell position during angiogenic sprouting.
Jakobsson L, Franco CA, Bentley K, Collins RT, Ponsioen B, Aspalter IM, Rosewell I, Busse M, Thurston G, Medvinsky A, Schulte-Merker S, Gerhardt H. | 11/13/2010 |
| Delta is endocytosed in a Neuralized-dependent manner from the basolateral membrane. | Neuralized promotes basal to apical transcytosis of delta in epithelial cells.
Benhra N, Vignaux F, Dussert A, Schweisguth F, Le Borgne R., Free PMC Article | 09/27/2010 |
| simulations of lateral inhibition incorporating Delta-Notch signaling by transient filopodial contacts between nonneighboring cells | Dynamic filopodia transmit intermittent Delta-Notch signaling to drive pattern refinement during lateral inhibition.
Cohen M, Georgiou M, Stevenson NL, Miodownik M, Baum B. | 09/6/2010 |
| Complementary patterns of Notch and Delta protein levels in early- and mid-stage embryos. | Notch and delta mRNAs in early-stage and mid-stage drosophila embryos exhibit complementary patterns of protein-producing potentials.
Shepherd A, Wesley U, Wesley C., Free PMC Article | 08/23/2010 |
| Data show that stl exhibits a strong mutant interaction with Delta (Dl), and Dl mutant ovaries show altered stl expression patterns. | Stall encodes an ADAMTS metalloprotease and interacts genetically with Delta in Drosophila ovarian follicle formation.
Ozdowski EF, Mowery YM, Cronmiller C., Free PMC Article | 04/19/2010 |
| The extracellular domain of Ser interacts with glycosphingolipids (GSL) in vitro via a conserved GSL-binding motif, raising the possibility that direct GSL-protein interactions modulate the endocytosis of Notch ligands. | Notch ligand activity is modulated by glycosphingolipid membrane composition in Drosophila melanogaster.
Hamel S, Fantini J, Schweisguth F., Free PMC Article | 03/29/2010 |
| suggests that within a NB lineage Delta is dispensable for sibling cell fate specification | On the roles of Notch, Delta, kuzbanian, and inscuteable during the development of Drosophila embryonic neuroblast lineages.
Udolph G, Rath P, Tio M, Toh J, Fang W, Pandey R, Technau GM, Chia W. | 01/21/2010 |
| In R1/R6/R7 photoreceptor precursors, unidirectional signaling depends on Dl's ability to inhibit Notch within the same cell. Loss of Dl-mediated cis-inhibition reverses the direction of lateral signaling. | cis-Inhibition of Notch by endogenous Delta biases the outcome of lateral inhibition.
Miller AC, Lyons EL, Herman TG., Free PMC Article | 01/21/2010 |
| inhibition and activation of Notch signaling rely on a conserved Neuralized-binding motif in Bearded proteins and the Notch ligand Delta | Both inhibition and activation of Notch signaling rely on a conserved Neuralized-binding motif in Bearded proteins and the Notch ligand Delta.
Fontana JR, Posakony JW., Free PMC Article | 01/21/2010 |