| In this study, we demonstrated that forced expression of Mix-like protein 1 (encoded by Mixl1) can be used to guide contribution of mouse embryonic stem cells to endodermal organs after blastocyst injection. | Targeted organ generation using Mixl1-inducible mouse pluripotent stem cells in blastocyst complementation.
Kobayashi T, Kato-Itoh M, Nakauchi H., Free PMC Article | 09/26/2015 |
| Embryonic localization of Mixl1 shows its co-localized with the early hematopoietic marker, Runx1, in the allantois and visceral yolk sac blood islands. | Mixl1 localizes to putative axial stem cell reservoirs and their posterior descendants in the mouse embryo.
Wolfe AD, Downs KM., Free PMC Article | 02/7/2015 |
| At later stages, in contrast with wild-type embryos, E7.5-8.0, Mixl1 knockout (KO) KO/KO embryos lacked morphologically recognizable midline structures such as the node and notochord. | A conditional mutant allele for analysis of Mixl1 function in the mouse.
Pulina MV, Sahr KE, Nowotschin S, Baron MH, Hadjantonakis AK., Free PMC Article | 01/10/2015 |
| Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity | Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity.
Pereira LA, Wong MS, Lim SM, Sides A, Stanley EG, Elefanty AG., Free PMC Article | 07/21/2012 |
| These data support the hypothesis that Mixl1 directly regulates Pdgfralpha and Flk1 gene expression. | Pdgfrα and Flk1 are direct target genes of Mixl1 in differentiating embryonic stem cells.
Pereira LA, Wong MS, Mossman AK, Sourris K, Janes ME, Knezevic K, Hirst CE, Lim SM, Pimanda JE, Stanley EG, Elefanty AG. | 05/12/2012 |
| results strongly suggest that Gsc is a direct target gene of Mixl1 during embryogenesis. | Transcriptional activation by the Mixl1 homeodomain protein in differentiating mouse embryonic stem cells.
Zhang H, Fraser ST, Papazoglu C, Hoatlin ME, Baron MH., Free PMC Article | 03/29/2010 |
| The studies suggest that high levels of Mixl1 preferentially allocate cells to the endoderm during ESC differentiation. | Enforced expression of Mixl1 during mouse ES cell differentiation suppresses hematopoietic mesoderm and promotes endoderm formation.
Lim SM, Pereira L, Wong MS, Hirst CE, Van Vranken BE, Pick M, Trounson A, Elefanty AG, Stanley EG. | 01/21/2010 |
| Overexpression of Mixl1 appears capable of inducing an abnormal self-renewal capacity in granulocytic precursors | The preleukemic state of mice reconstituted with Mixl1-transduced marrow cells.
Metcalf D, Glaser S, Mifsud S, Di Rago L, Robb L., Free PMC Article | 01/21/2010 |
| As goosecoid is itself induced in a Foxh1-dependent manner, we propose that Foxh1 initiates positive and negative transcriptional circuits to refine cell fate decisions during gastrulation | Foxh1 recruits Gsc to negatively regulate Mixl1 expression during early mouse development.
Izzi L, Silvestri C, von Both I, Labbé E, Zakin L, Wrana JL, Attisano L., Free PMC Article | 01/21/2010 |
| non-cell autonomous role in nascent mesoderm in the formation and/or patterning of adjacent definitive endoderm. | Mouse Mix gene is activated early during differentiation of ES and F9 stem cells and induces endoderm in frog embryos.
Mohn D, Chen SW, Dias DC, Weinstein DC, Dyer MA, Sahr K, Ducker CE, Zahradka E, Keller G, Zaret KS, Gudas LJ, Baron MH. | 01/21/2010 |
| all mice reconstituted with Mixl1-transduced bone marrow developed fatal, transplantable acute myeloid leukemia with a mean latency period of 200 days | Enforced expression of the homeobox gene Mixl1 impairs hematopoietic differentiation and results in acute myeloid leukemia.
Glaser S, Metcalf D, Wu L, Hart AH, DiRago L, Mifsud S, D'Amico A, Dagger S, Campo C, Chan AC, Izon DJ, Robb L., Free PMC Article | 01/21/2010 |
| Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells | The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells.
Ng ES, Azzola L, Sourris K, Robb L, Stanley EG, Elefanty AG. | 01/21/2010 |
| required for the morphogenesis of axial mesoderm, the heart and the gut during embryogenesis | Mixl1 is required for axial mesendoderm morphogenesis and patterning in the murine embryo.
Hart AH, Hartley L, Sourris K, Stadler ES, Li R, Stanley EG, Tam PP, Elefanty AG, Robb L. | 01/21/2010 |