| Using in vitro pull-down experiments and density glycerol gradients, the authors found that at least 3 regions distributed over its entire length mediate the self-interaction of mouse hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus (SARS-CoV) N protein. | Coronavirus nucleocapsid proteins assemble constitutively in high molecular oligomers.
Cong Y, Kriegenburg F, de Haan CAM, Reggiori F., Free PMC Article | 01/26/2019 |
| The severe acute respiratory syndrome coronavirus N protein was found to bind to the SPRY domain of the tripartite motif protein 25 (TRIM25) E3 ubiquitin ligase, thereby interfering with the association between TRIM25 and retinoic acid-inducible gene I (RIG-I) and inhibiting TRIM25-mediated RIG-I ubiquitination and activation. | The Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Inhibits Type I Interferon Production by Interfering with TRIM25-Mediated RIG-I Ubiquitination.
Hu Y, Li W, Gao T, Cui Y, Jin Y, Li P, Ma Q, Liu X, Cao C., Free PMC Article | 05/20/2017 |
| Electrostatic repulsion acts as a switch to regulate SARS virus N protein oligomerization. | Transient oligomerization of the SARS-CoV N protein--implication for virus ribonucleoprotein packaging.
Chang CK, Chen CM, Chiang MH, Hsu YL, Huang TH., Free PMC Article | 01/11/2014 |
| These results suggest that SARS coronavirus could reduce pyruvate kinase activity via its nucleocapsid protein, and this may in turn cause disease. | SARS-CoV nucleocapsid protein interacts with cellular pyruvate kinase protein and inhibits its activity.
Wei WY, Li HC, Chen CY, Yang CH, Lee SK, Wang CW, Ma HC, Juang YL, Lo SY., Free PMC Article | 05/19/2012 |
| The co-localization of SARS-CoVN and CXCL16 in the cytoplasm of HEK293FT cells was also shown using confocal laser scanning microscopy. | Cxcl16 interact with SARS-CoV N protein in and out cell.
Zhang YP, Zhang RW, Chang WS, Wang YY., Free PMC Article | 01/29/2011 |
| The HLA-A 2402 complexed with SARSV N1 showed a novel host strategy to present an immunodominant CTL epitope by intrachain hydrogen bond as a featured epitope. | Novel immunodominant peptide presentation strategy: a featured HLA-A*2402-restricted cytotoxic T-lymphocyte epitope stabilized by intrachain hydrogen bonds from severe acute respiratory syndrome coronavirus nucleocapsid protein.
Liu J, Wu P, Gao F, Qi J, Kawana-Tachikawa A, Xie J, Vavricka CJ, Iwamoto A, Li T, Gao GF., Free PMC Article | 11/27/2010 |
| SARS-CoV N interacts with MAP19 and increased the expression of MAP19 in cells. | [Study on interaction between SARS-CoV N and MAP19].
Liu JL, Cao C, Ma QJ. | 03/8/2010 |
| SARS-CoV N protein specifically modulates transcription of the FGL2 gene to cause fibrosis and vascular thrombosis. | Severe acute respiratory syndrome coronavirus nucleocapsid protein does not modulate transcription of the human FGL2 gene.
Siu KL, Chan CP, Chan C, Zheng BJ, Jin DY. | 01/21/2010 |
| SR-rich motif is critical for effective virus replication. | The SR-rich motif in SARS-CoV nucleocapsid protein is important for virus replication.
Tylor S, Andonov A, Cutts T, Cao J, Grudesky E, Van Domselaar G, Li X, He R. | 01/21/2010 |
| thermostability of the N-terminal RNA-binding domain (RBD) of the N protein; results showed the thermal-induced unfolding-folding transition of the RBD follows a two-state model with a reversibility >90% | Thermostability of the N-terminal RNA-binding domain of the SARS-CoV nucleocapsid protein: experiments and numerical simulations.
Fang HJ, Chen YZ, Li MS, Wu MC, Chang CL, Chang CK, Hsu YL, Huang TH, Chen HM, Tsong TY, Hu CK., Free PMC Article | 01/21/2010 |
| The SARS-CoV N protein is a modular protein containing multiple RNA-binding sites. | Multiple nucleic acid binding sites and intrinsic disorder of severe acute respiratory syndrome coronavirus nucleocapsid protein: implications for ribonucleocapsid protein packaging.
Chang CK, Hsu YL, Chang YH, Chao FA, Wu MC, Huang YS, Hu CK, Huang TH., Free PMC Article | 01/21/2010 |
| the domain conferring the ability to direct virus-like particle assembly and release in SARS-CoV N is largely contained between residues 168 and 421 | Severe acute respiratory syndrome coronavirus nucleocapsid protein confers ability to efficiently produce virus-like particles when substituted for the human immunodeficiency virus nucleocapsid domain.
Wang SM, Chang YF, Chen YM, Wang CT., Free PMC Article | 01/21/2010 |
| An excessive host immune response against the nucleocapsid protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is involved in severe pneumonia caused by SARS-CoV infection. | Prior immunization with severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV.
Yasui F, Kai C, Kitabatake M, Inoue S, Yoneda M, Yokochi S, Kase R, Sekiguchi S, Morita K, Hishima T, Suzuki H, Karamatsu K, Yasutomi Y, Shida H, Kidokoro M, Mizuno K, Matsushima K, Kohara M. | 01/21/2010 |
| both E and N proteins must be coexpressed with M protein for the efficient production and release of virus-like particles by transfected Vero E6 cells. | The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particles.
Siu YL, Teoh KT, Lo J, Chan CM, Kien F, Escriou N, Tsao SW, Nicholls JM, Altmeyer R, Peiris JS, Bruzzone R, Nal B., Free PMC Article | 01/21/2010 |
| This paper describes the work to identify two proteins/protein fragments of N protein and to determine their source. | SARS coronavirus: unusual lability of the nucleocapsid protein.
Mark J, Li X, Cyr T, Fournier S, Jaentschke B, Hefford MA., Free PMC Article | 01/21/2010 |
| fgl2 gene transcription induced by the N protein of SARS-CoV was dependent on transcription factor C/EBP alpha binding with its cognate cis-element in fgl2 promoter. | The nucleocapsid protein of SARS-CoV induces transcription of hfgl2 prothrombinase gene dependent on C/EBP alpha.
Han M, Yan W, Huang Y, Yao H, Wang Z, Xi D, Li W, Zhou Y, Hou J, Luo X, Ning Q., Free PMC Article | 01/21/2010 |
| Phosphorylation of the arginine/serine motif of the nucleocapsid protein did not significantly affect RNA binding of the nucleocapsid protein but impaired its multimerization ability. | Phosphorylation of the arginine/serine dipeptide-rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization.
Peng TY, Lee KR, Tarn WY., Free PMC Article | 01/21/2010 |
| This study employed the SAIL method to determine the high-quality solution structure of the severe acute respiratory syndrome coronavirus nucleocapsid protein C-terminal domain by NMR. | Solution structure of the c-terminal dimerization domain of SARS coronavirus nucleocapsid protein solved by the SAIL-NMR method.
Takeda M, Chang CK, Ikeya T, Güntert P, Chang YH, Hsu YL, Huang TH, Kainosho M., Free PMC Article | 01/21/2010 |
| It was demonstrated that the N protein of SARS-CoV induces aggregation of EF1, inhibiting protein translation and cytokinesis by blocking F-actin bundling. | The nucleocapsid protein of severe acute respiratory syndrome coronavirus inhibits cell cytokinesis and proliferation by interacting with translation elongation factor 1alpha.
Zhou B, Liu J, Wang Q, Liu X, Li X, Li P, Ma Q, Cao C., Free PMC Article | 01/21/2010 |
| SARS coronavirus nucleocapsid protein interacts with Smad3 and modulates transforming growth factor-beta signaling | Severe acute respiratory syndrome-associated coronavirus nucleocapsid protein interacts with Smad3 and modulates transforming growth factor-beta signaling.
Zhao X, Nicholls JM, Chen YG., Free PMC Article | 01/21/2010 |
| The experiments revealed that N induces the intrinsic apoptotic pathway, resulting in processing of N at residues 400 and 403 by caspase-6 and/or caspase-3. | Cell type-specific cleavage of nucleocapsid protein by effector caspases during SARS coronavirus infection.
Diemer C, Schneider M, Seebach J, Quaas J, Frösner G, Schätzl HM, Gilch S., Free PMC Article | 01/21/2010 |
| Similar levels of IgG responses to the S and N proteins were observed in SARS patients during the manifestation and convalescent stages; In the postinfection period, patients had much lower serum IgG levels against S450-650 than against the N protein | Comparison of immunoglobulin G responses to the spike and nucleocapsid proteins of severe acute respiratory syndrome (SARS) coronavirus in patients with SARS.
Zhao J, Wang W, Wang W, Zhao Z, Zhang Y, Lv P, Ren F, Gao XM., Free PMC Article | 01/21/2010 |
| We also showed that N protein activated IL-6 expression through NF-kappaB by facilitating the translocation of NF-kappaB from cytosol to nucleus. | Nucleocapsid protein of SARS-CoV activates interleukin-6 expression through cellular transcription factor NF-kappaB.
Zhang X, Wu K, Wang D, Yue X, Song D, Zhu Y, Wu J., Free PMC Article | 01/21/2010 |
| Binding to cyclophilin A during invasion of host cells. | Function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome coronavirus.
Chen Z, Mi L, Xu J, Yu J, Wang X, Jiang J, Xing J, Shang P, Qian A, Li Y, Shaw PX, Wang J, Duan S, Ding J, Fan C, Zhang Y, Yang Y, Yu X, Feng Q, Li B, Yao X, Zhang Z, Li L, Xue X, Zhu P., Free PMC Article | 01/21/2010 |
| the nucleocapsid protein of severe acute respiratory syndrome coronavirus is sumoylated by interaction with Ubc9 | Sumoylation of the nucleocapsid protein of severe acute respiratory syndrome coronavirus by interaction with Ubc9.
Li Q, Xiao H, Tam JP, Liu DX., Free PMC Article | 01/21/2010 |