| Prevention of age-related truncation of gamma-glutamylcysteine ligase catalytic subunit (GCLC) delays cataract formation. | Prevention of age-related truncation of γ-glutamylcysteine ligase catalytic subunit (GCLC) delays cataract formation.
Wei Z, Hao C, Radeen KR, Srinivasagan R, Chen JK, Sharma S, McGee-Lawrence ME, Hamrick MW, Monnier VM, Fan X., Free PMC Article | 04/30/2024 |
| Cystine/Glutamate Xc[-] Antiporter Induction Compensates for Transsulfuration Pathway Repression by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) to Ensure Cysteine for Hepatic Glutathione Biosynthesis. | Cystine/Glutamate Xc(-) Antiporter Induction Compensates for Transsulfuration Pathway Repression by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) to Ensure Cysteine for Hepatic Glutathione Biosynthesis.
Orlowska K, Fling RR, Nault R, Schilmiller AL, Zacharewski TR., Free PMC Article | 06/21/2023 |
| Glutathione-dependent redox balance characterizes the distinct metabolic properties of follicular and marginal zone B cells. | Glutathione-dependent redox balance characterizes the distinct metabolic properties of follicular and marginal zone B cells.
Franchina DG, Kurniawan H, Grusdat M, Binsfeld C, Guerra L, Bonetti L, Soriano-Baguet L, Ewen A, Kobayashi T, Farinelle S, Minafra AR, Vandamme N, Carpentier A, Borgmann FK, Jäger C, Chen Y, Kleinewietfeld M, Vasiliou V, Mittelbronn M, Hiller K, Lang PA, Brenner D., Free PMC Article | 04/23/2022 |
| Carnosic Acid Attenuates an Early Increase in ROS Levels during Adipocyte Differentiation by Suppressing Translation of Nox4 and Inducing Translation of Antioxidant Enzymes. | Carnosic Acid Attenuates an Early Increase in ROS Levels during Adipocyte Differentiation by Suppressing Translation of Nox4 and Inducing Translation of Antioxidant Enzymes.
Lee DK, Jang HD., Free PMC Article | 07/17/2021 |
| The development of glutamate cysteine ligase [GCL] transgenic and gene deletion mouse lines are described. | Glutamate cysteine ligase (GCL) transgenic and gene-targeted mice for controlling glutathione synthesis.
Mohar I, Botta D, White CC, McConnachie LA, Kavanagh TJ. | 04/10/2020 |
| The findings indicate that expression of the transcription factor NRF2 and its effector GCL are both profoundly deregulated in endometriotic lesions towards increased growth and fibrogenetic processes. | Alteration of Nrf2 and Glutamate Cysteine Ligase expression contribute to lesions growth and fibrogenesis in ectopic endometriosis.
Marcellin L, Santulli P, Chouzenoux S, Cerles O, Nicco C, Dousset B, Pallardy M, Kerdine-Römer S, Just PA, Chapron C, Batteux F. | 04/14/2018 |
| Retinal GCLC was significantly increased in rd10 mice at P21 as well as GSSG. Our results suggest alterations in retinal GCLC content and GSH and/or its precursors in these two RP animal models. Regulation of the enzymes related to GSH metabolism and the retinal concentration of glutamate may be a possible target to delay especially cone death in Retinitis Pigmentosa | Alterations in glutamate cysteine ligase content in the retina of two retinitis pigmentosa animal models.
Sánchez-Vallejo V, Benlloch-Navarro S, Trachsel-Moncho L, López-Pedrajas R, Almansa I, Romero FJ, Miranda M. | 01/13/2018 |
| Data show that the catalytic subunit of glutamate cysteine ligase (Gclc)-derived glutathione buffers reactive oxygen species (ROS), and regulates metabolic reprogramming. | Glutathione Primes T Cell Metabolism for Inflammation.
Mak TW, Grusdat M, Duncan GS, Dostert C, Nonnenmacher Y, Cox M, Binsfeld C, Hao Z, Brüstle A, Itsumi M, Jäger C, Chen Y, Pinkenburg O, Camara B, Ollert M, Bindslev-Jensen C, Vasiliou V, Gorrini C, Lang PA, Lohoff M, Harris IS, Hiller K, Brenner D. | 06/3/2017 |
| To study the biological effects of low GSH levels, we disrupted its synthesis both at birth by breeding a Gclc loxP mouse with a thy1-cre mouse and at a later age by breeding with a CaMKII-ERT2-Cre (FIGSKO mouse). FIGSKO mice also develop cognitive abnormalities, i.e. learning impairment and nesting behaviors based on passive avoidance, T-Maze, and nesting behavior tests | Gclc deficiency in mouse CNS causes mitochondrial damage and neurodegeneration.
Feng W, Rosca M, Fan Y, Hu Y, Feng P, Lee HG, Monnier VM, Fan X., Free PMC Article | 05/20/2017 |
| A floxed Gclc mouse was generated and crossed with a transgenic mouse expressing Cre in the lens to generate the Lens Glutathione Synthesis Knockout mouse in which de novo GSH synthesis was completely abolished in the lens. | The LEGSKO mouse: a mouse model of age-related nuclear cataract based on genetic suppression of lens glutathione synthesis.
Fan X, Liu X, Hao S, Wang B, Robinson ML, Monnier VM., Free PMC Article | 06/1/2013 |
| Clinically relevant levels of TGF-beta1 suppresses GCLC and GCLM expression in mouse lung. | Transforming growth factor β suppresses glutamate-cysteine ligase gene expression and induces oxidative stress in a lung fibrosis model.
Liu RM, Vayalil PK, Ballinger C, Dickinson DA, Huang WT, Wang S, Kavanagh TJ, Matthews QL, Postlethwait EM., Free PMC Article | 01/26/2013 |
| Data show for the first time that GCLC may serve a dual role, as a surrogate marker for cellular redox state as well as malignant potential of melanoma cells. | High expression of GCLC is associated with malignant melanoma of low oxidative phenotype and predicts a better prognosis.
Mougiakakos D, Okita R, Ando T, Dürr C, Gadiot J, Ichikawa J, Zeiser R, Blank C, Johansson CC, Kiessling R. | 11/17/2012 |
| The impacts of four clinical missense mutations on GCLC enzymatic function in vivo and in vitro, was evaluated. | Enzymatic defects underlying hereditary glutamate cysteine ligase deficiency are mitigated by association of the catalytic and regulatory subunits.
Willis MN, Liu Y, Biterova EI, Simpson MA, Kim H, Lee J, Barycki JJ., Free PMC Article | 09/24/2011 |
| tBHQ has beneficial effects on reducing hyperglycemia-induced kidney injury, which is associated with the enhanced expression of Nrf2, and its downstream antioxidant HO-1 and gamma-GCS in the glomeruli of diabetic mice | Attenuation of glomerular injury in diabetic mice with tert-butylhydroquinone through nuclear factor erythroid 2-related factor 2-dependent antioxidant gene activation.
Li H, Zhang L, Wang F, Shi Y, Ren Y, Liu Q, Cao Y, Duan H. | 09/3/2011 |
| In first days of life luminescence measured was in all mice with distinct strain differences indicating NF-kappaB, superoxide dismutase, gamma-glutamylcysteine synthetase, and antioxidant responsive element activity. | Antioxidant activity in the newborn brain: a luciferase mouse model.
Dohlen G, Odland HH, Carlsen H, Blomhoff R, Thaulow E, Saugstad OD. | 03/8/2011 |
| Hypoxia decreased 2 key enzyme activities that regulate GSH synthesis, glutamate cysteine ligase (GCL) (E.C. 6.3.2.2) and glutathione synthase (GS) (E.C. 6.3.2.3) | Hypoxia and kinase activity regulate lung epithelial cell glutathione.
Jackson RM, Gupta C. | 05/31/2010 |
| activation of GSK-3beta is a key mediator of the initial phase of acetaminophen-induced liver injury through modulating GCL and Mcl-1 degradation, as well as JNK activation in liver | Silencing glycogen synthase kinase-3beta inhibits acetaminophen hepatotoxicity and attenuates JNK activation and loss of glutamate cysteine ligase and myeloid cell leukemia sequence 1.
Shinohara M, Ybanez MD, Win S, Than TA, Jain S, Gaarde WA, Han D, Kaplowitz N., Free PMC Article | 04/19/2010 |
| These findings demonstrate that TAT-mediated transduction of wild-type or dominant-inhibitory mutants of the Glutamate cysteine ligase (GCL) subunits is a viable means of manipulating cellular GCL activity. | Manipulation of cellular GSH biosynthetic capacity via TAT-mediated protein transduction of wild-type or a dominant-negative mutant of glutamate cysteine ligase alters cell sensitivity to oxidant-induced cytotoxicity.
Backos DS, Brocker CN, Franklin CC., Free PMC Article | 03/15/2010 |
| Homocysteine stimulates antioxidant response element-mediated expression of glutamate-cysteine ligase in mouse macrophages | Homocysteine stimulates antioxidant response element-mediated expression of glutamate-cysteine ligase in mouse macrophages.
Bea F, Hudson FN, Neff-Laford H, White CC, Kavanagh TJ, Kreuzer J, Preusch MR, Blessing E, Katus HA, Rosenfeld ME., Free PMC Article | 01/21/2010 |
| These results suggest that intense noise exposure facilitates the expression of GCL catalytic subunit in the cochlea possibly through the activation of transcription factors including AP-1 and NF-kappaB. | Acoustic overstimulation facilitates the expression of glutamate-cysteine ligase catalytic subunit probably through enhanced DNA binding of activator protein-1 and/or NF-kappaB in the murine cochlea.
Nagashima R, Sugiyama C, Yoneyama M, Kuramoto N, Kawada K, Ogita K. | 01/21/2010 |
| The Gclc, the gene that encodes the catalytic subunit of the enzyme glutamate cysteine ligase, was used as a molecular surrogate for investigating the mechanisms by which TGF-beta suppressed Phase II gene expression. | Smad3-ATF3 signaling mediates TGF-beta suppression of genes encoding Phase II detoxifying proteins.
Bakin AV, Stourman NV, Sekhar KR, Rinehart C, Yan X, Meredith MJ, Arteaga CL, Freeman ML. | 01/21/2010 |
| glutamate-cysteine ligase activity is an important factor in determining acetaminophen-induced liver injury in C57Bl/6 male mice | Acetaminophen-induced liver injury is attenuated in male glutamate-cysteine ligase transgenic mice.
Botta D, Shi S, White CC, Dabrowski MJ, Keener CL, Srinouanprachanh SL, Farin FM, Ware CB, Ladiges WC, Pierce RH, Fausto N, Kavanagh TJ. | 01/21/2010 |
| Results of this study suggest that mechanisms of modulation of eukaryotic GCL enzymes may include specific binding of ligands such as pyridine dinucleotide phosphates and reversible protein phosphorylation. | Mechanisms of gamma-glutamylcysteine ligase regulation.
Toroser D, Yarian CS, Orr WC, Sohal RS., Free PMC Article | 01/21/2010 |
| study demonstrates that platelet-derived growth factor increases transcription of the gamma-glutamylcysteine synthetase heavy subunit in NIH 3T3 fibroblasts via H2O2 and activation of protein kinase C | The role of H2O2 in the platelet-derived growth factor-induced transcription of the gamma-glutamylcysteine synthetase heavy subunit.
Catarzi S, Degl'Innocenti D, Iantomasi T, Favilli F, Vincenzini MT., Free PMC Article | 01/21/2010 |
| The GCLc protein was widely expressed throughout the embryo, and, in general, it co-localized with the Gclc mRNA. | Expression of glutamate-cysteine ligase during mouse development.
Díaz D, Krejsa CM, Kavanagh TJ. | 01/21/2010 |