WHI3 mRNA-binding protein WHI3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: WHI3
Official Full Name: mRNA-binding protein WHI3
Primary source: SGD:S000005141
Locus tag: YNL197C
See related: AllianceGenome:SGD:S000005141; FungiDB:YNL197C; VEuPathDB:YNL197C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mRNA binding activity. Involved in several processes, including invasive growth in response to glucose limitation; pseudohyphal growth; and traversing start control point of mitotic cell cycle. Located in P-body; cytoplasmic stress granule; and endoplasmic reticulum membrane. Orthologous to human RBPMS (RNA binding protein, mRNA processing factor) and RBPMS2 (RNA binding protein, mRNA processing factor 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See WHI3 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (267608..269593, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

aging phenotypes previously attributed to loss of heterochromatin silencing are instead caused by aggregation of the Whi3 cell cycle regulator.
Title: Aggregation of the Whi3 protein, not loss of heterochromatin, causes sterility in old yeast cells.
In CLN3, Whi3 may bind to the 3' GCAU motifs to attract the Ccr4-Not complex to promote RNA deadenylation and turnover, and Whi3 may bind to the 5' GCAU motifs to inhibit translation.
Title: Effects of the yeast RNA-binding protein Whi3 on the half-life and abundance of CLN3 mRNA and other targets.
an expanded model for Whi3 function
Title: Whi3, an S. cerevisiae RNA-binding protein, is a component of stress granules that regulates levels of its target mRNAs.
Hog1 activity is required for restart from G1 arrest under osmotic stress conditions, whereas Whi3 acts as a negative regulator for this restart mechanism
Title: Evidence of antagonistic regulation of restart from G(1) delay in response to osmotic stress by the Hog1 and Whi3 in budding yeast.
Whi3 controls ploidy, developmental signaling and production of cyclins Cln1/Cln2.
Title: The RNA-binding protein Whi3 is a key regulator of developmental signaling and ploidy in Saccharomyces cerevisiae.
Whi3 liberates the G1 cyclin Cln3 from translational inhibition. Super-assembly of Whi3 is a slow response to pheromone, driven by polyQ and polyN domains, counteracted by Hsp70, and stable over generations. Unlike prion aggregates, Whi3 super-assemblies are not inherited mitotically but segregate to the mother cell.
Title: A super-assembly of Whi3 encodes memory of deceptive encounters by single cells during yeast courtship.
Whi3 is required for the apical localization of Cdc28-Cln1,2 complexes during bud growth and thereby, to promote the activation of Cdc42 and its effectors in the bud apex.
Title: Whi3 regulates morphogenesis in budding yeast by enhancing Cdk functions in apical growth.
Whi3 is a general modulator of protein fate in budding yeast
Title: Whi3, a developmental regulator of budding yeast, binds a large set of mRNAs functionally related to the endoplasmic reticulum.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in invasive growth in response to glucose limitation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pseudohyphal growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell size	HMP more info	PubMed
involved_in regulation of mRNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in traversing start control point of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in traversing start control point of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: mRNA-binding protein WHI3

NP_014202.1

RNA binding protein that modulates mRNA stability; regulates cell cycle, sister chromatid cohesion, and stress response genes; modulates stability and translational efficiency of CLN3 mRNA; colocalizes with P-bodies/stress granules to regulate target mRNAs; regulates cell fate and critical cell size for Start passage; regulates ploidy; self-templating mnemon that forms inactive super-assemblies, preventing G1 arrest in mother cells after unsuccessful mating and causing sterility in old cells

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

267608..269593 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183035.1 → NP_014202.1 TPA: mRNA-binding protein WHI3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014202.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0Z0, P34761

UniProtKB/TrEMBL

A6ZRP0, N1NXA0

Conserved Domains (2) summary

COG0724
Location:450 → 648

RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]

cd12245
Location:536 → 624

RRM_scw1_like; RNA recognition motif (RRM) found in yeast cell wall integrity protein scw1 and similar proteins