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    TOC75-III translocon at the outer envelope membrane of chloroplasts 75-III [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 823827, updated on 18-Sep-2024

    Summary

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    Official Symbol
    TOC75-III
    Official Full Name
    translocon at the outer envelope membrane of chloroplasts 75-III
    Primary source
    TAIR:AT3G46740
    Locus tag
    AT3G46740
    See related
    Araport:AT3G46740
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    MAR1; MODIFIER OF ARG1 1; translocon at the outer envelope membrane of chloroplasts 75-III
    Summary
    Component of the translocon outer membrane (TOC) complex. Forms the outer envelope translocation channel (beta-barrel). Plays a role in preprotein conductance. Imported into chloroplast. Expressed in young dividing photosynthetic tissues. Knockout mutants are embryo lethal with arrested development at the two-cell stage. Knockout mutants have abnormal etioplasts.
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    Genomic context

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    See TOC75-III in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    7
    Sequence:
    Chromosome: 3; NC_003074.8 (17215813..17219411, complement)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene UDP-Glycosyltransferase superfamily protein Neighboring gene NB-ARC domain-containing disease resistance protein Neighboring gene uncharacterized protein Neighboring gene low-temperature-induced protein Neighboring gene Protein kinase superfamily protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts. Chen YL, et al. Plant Physiol, 2016 Sep. PMID 27388682, Free PMC Article
    2. Chloroplast Omp85 proteins change orientation during evolution. Sommer MS, et al. Proc Natl Acad Sci U S A, 2011 Aug 16. PMID 21825140, Free PMC Article
    3. Complete maturation of the plastid protein translocation channel requires a type I signal peptidase. Inoue K, et al. J Cell Biol, 2005 Nov 7. PMID 16275749, Free PMC Article
    4. In vivo analyses of the roles of essential Omp85-related proteins in the chloroplast outer envelope membrane. Huang W, et al. Plant Physiol, 2011 Sep. PMID 21757633, Free PMC Article
    5. Functional analysis of the two Arabidopsis homologues of Toc34, a component of the chloroplast protein import apparatus. Gutensohn M, et al. Plant J, 2000 Sep. PMID 10998188

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Toc75 polypeptide transport-associated (POTRA) domains are located in the intermembrane space of the chloroplast envelope by immunogold electron microscopy.
      Title: Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts.
    2. Data indicate that OEP80-T7(atToc75-V) and atToc75-III showed distinct migration patterns on blue native-PAGE.
      Title: OEP80, an essential protein paralogous to the chloroplast protein translocation channel Toc75, exists as a 70-kD protein in the Arabidopsis thaliana chloroplast outer envelope.
    3. the POTRA domains of both Toc75-III and Toc75-V are exposed to the cytoplasm
      Title: Chloroplast Omp85 proteins change orientation during evolution.
    4. SPase I converted Toc75 precursor to its mature form in vitro.
      Title: Complete maturation of the plastid protein translocation channel requires a type I signal peptidase.
    5. Embryo development in the atToc75-III mutant, lacking the pore forming component of the translocase, was arrested extremely early at the two-cell stage.
      Title: Deletion of core components of the plastid protein import machinery causes differential arrest of embryo development in Arabidopsis thaliana.
    6. atTOC75-III of the Toc75 (translocon at the outer envelope membrane of chloroplasts, 75 kD) gene family is essential for plant viability, and is expressed at its highest level in young, rapidly expanding tissues
      Title: A molecular-genetic study of the Arabidopsis Toc75 gene family.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_114541.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein-transporting ATPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within chloroplast organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within chloroplast organization ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    acts_upstream_of_or_within embryonic morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in intracellular protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within protein import into chloroplast stroma IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein targeting to chloroplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of TOC-TIC supercomplex I IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of Toc complex ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    located_in chloroplast HDA PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast envelope HDA PubMed 
    located_in chloroplast outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    located_in plant-type vacuole HDA PubMed 
    located_in plastid HDA PubMed 

    General protein information

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    Preferred Names
    translocon at the outer envelope membrane of chloroplasts 75-III
    NP_190258.1
    • translocon at the outer envelope membrane of chloroplasts 75-III (TOC75-III); FUNCTIONS IN: P-P-bond-hydrolysis-driven protein transmembrane transporter activity; INVOLVED IN: protein targeting to chloroplast, chloroplast organization, embryonic morphogenesis; LOCATED IN: in 6 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Bacterial surface antigen (D15) (InterPro:IPR000184), Chloroplast envelope protein translocase, IAP75 (InterPro:IPR005689), Surface antigen variable number (InterPro:IPR010827); BEST Arabidopsis thaliana protein match is: Outer membrane OMP85 family protein (TAIR:AT4G09080.1); Has 4030 Blast hits to 1859 proteins in 343 species: Archae - 0; Bacteria - 806; Metazoa - 1937; Fungi - 82; Plants - 633; Viruses - 22; Other Eukaryotes - 550 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      17215813..17219411 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_114541.3 → NP_190258.1  translocon at the outer envelope membrane of chloroplasts 75-III [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_190258.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9STE8
      UniProtKB/TrEMBL
      A0A178VEL0
      Conserved Domains (2) summary
      PLN03138
      Location:8 → 818
      PLN03138; Protein TOC75; Provisional
      pfam01103
      Location:486 → 815
      Bac_surface_Ag; Surface antigen
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9STE8.1

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