ninaE neither inactivation nor afterpotential E [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: ninaEprovided by FlyBase
Official Full Name: neither inactivation nor afterpotential Eprovided by FlyBase
Primary source: FLYBASE:FBgn0002940
Locus tag: Dmel_CG4550
See related: AllianceGenome:FB:FBgn0002940
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 143283_at; 1F9; BEST:GH11778; CG4550; Dm Rh1; DMELRH1; Dmel\CG4550; DmRh1; DRh1; Nina E; NinaE; opsin; ora; R; r1; Rh; Rh-1; rh1; Rh1; RH1; Rh1(ninaE); rh1/ninaE; Rh1/ninaE; rhodopsin; Rhodopsin-1
Summary: Enables G protein-coupled photoreceptor activity. Involved in several processes, including detection of light stimulus; negative regulation of compound eye retinal cell programmed cell death; and photoreceptor cell development. Located in early endosome and subrhabdomeral cisterna. Part of inaD signaling complex. Colocalizes with perinuclear endoplasmic reticulum. Is expressed in adult head; photoreceptor; and photoreceptor cell. Used to study retinal degeneration; retinal disease; retinitis pigmentosa; and retinitis pigmentosa 4. Orthologous to human OPN4 (opsin 4). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 92B4-92B4; 3-67 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (19886255..19888206, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (15711977..15713928, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

we report the identification of a retinoid-inducible gene in Drosophila, highroad, which is required for photoreceptors to clear folding-defective mutant Rhodopsin-1 proteins
Title: highroad Is a Carboxypetidase Induced by Retinoids to Clear Mutant Rhodopsin-1 in Drosophila Retinitis Pigmentosa Models.
These data demonstrate that the V1 and V0 components of V-ATPase play key roles in post-Golgi trafficking of Rh1.
Title: The V-ATPase V1 subunit A1 is required for rhodopsin anterograde trafficking in Drosophila.
The Rh1(also known as NinaE))-trafficking defect in PIP4K-depleted cells could be suppressed by downregulating Rab5 function or by selectively reconstituting PIP4K in the PI3P-enriched early endosomal compartment of photoreceptors
Title: Phosphatidylinositol 5-phosphate 4-kinase regulates early endosomal dynamics during clathrin-mediated endocytosis.
Organisms use multiple strategies to maximize visual capabilities in different light conditions. Many invertebrates show a daily cycle of shedding the photoreceptor's rhabdomeric membranes at dawn and rebuilding these during the following night. We show here that the Aedes aegypti mosquito possesses two distinct light-driven cellular signaling processes for modulating rhodopsin content during this cycle.
Title: Light-Driven Processes Control Both Rhodopsin Maturation and Recycling in Mosquito Photoreceptors.
Fmr1 protein associates with ninaE mRNA and represses its translation.
Title: Bidirectional regulation of fragile X mental retardation protein phosphorylation controls rhodopsin homoeostasis.
Data show that site-directed mutagenesis of conserved residues has only modest effects on Rhodopsin 1 absorption.
Title: Analysis of Conserved Glutamate and Aspartate Residues in Drosophila Rhodopsin 1 and Their Influence on Spectral Tuning.
Data indicate that mutations in Golgi SNARE protein gos28 lead to defective rhodopsin (Rh1) trafficking.
Title: The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival.
Upon light stimulation, Crag is required for trafficking of Rh from the trans-Golgi network to rhabdomere membranes via a Rab11-dependent vesicular transport.
Title: Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells.
GPI biosynthesis is essential for rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors
Title: GPI biosynthesis is essential for rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors.
Our results indicate that fatp promotes photoreceptor survival by regulating Rh1 abundance
Title: Drosophila fatty acid transport protein regulates rhodopsin-1 metabolism and is required for photoreceptor neuron survival.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm1706 (e-PCR)
- UniSTS:48866

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables G protein-coupled photoreceptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled photoreceptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables G protein-coupled photoreceptor activity	NAS Non-traceable Author Statement more info	PubMed
enables G protein-coupled receptor activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in adult locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to light stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to light stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in detection of UV	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in detection of visible light	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of compound eye retinal cell programmed cell death	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in optomotor response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipase C-activating opsin-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in photoreceptor cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phototaxis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phototransduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in phototransduction	IEA Inferred from Electronic Annotation more info
involved_in phototransduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phototransduction	NAS Non-traceable Author Statement more info	PubMed
involved_in response to light intensity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rhabdomere development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in thermotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in visual perception	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
part_of inaD signaling complex	IPI Inferred from Physical Interaction more info	PubMed
part_of inaD signaling complex	TAS Traceable Author Statement more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
colocalizes_with perinuclear endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in rhabdomere	IDA Inferred from Direct Assay more info	PubMed
located_in subrhabdomeral cisterna	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: neither inactivation nor afterpotential E

Names: CG4550-PA; ninaE-PA; rhodopsin; rhodopsin 1; rhodopsin-1; rhodopsin1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

19886255..19888206 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079683.3 → NP_524407.1 neither inactivation nor afterpotential E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524407.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A0AVV9, P06002, Q4QPQ2, Q9TX56, Q9VDS8

Related

FBpp0083266

Conserved Domains (1) summary

cd15079
Location:52 → 340

7tmA_photoreceptors_insect; insect photoreceptors R1-R6 and similar proteins, member of the class A family of seven-transmembrane G protein-coupled receptors