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    Hakai hakai [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 35256, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Hakaiprovided by FlyBase
    Official Full Name
    hakaiprovided by FlyBase
    Primary source
    FLYBASE:FBgn0032812
    Locus tag
    Dmel_CG10263
    See related
    AllianceGenome:FB:FBgn0032812
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG10263; Dmel\CG10263; hakai
    Summary
    Enables cadherin binding activity. Involved in several processes, including embryonic development via the syncytial blastoderm; epithelial cell migration, open tracheal system; and regulation of alternative mRNA splicing, via spliceosome. Located in several cellular components, including cytoplasmic vesicle; cytosol; and perinuclear region of cytoplasm. Part of RNA N6-methyladenosine methyltransferase complex. Is expressed in endoderm; endoderm anlage; and organism. Orthologous to human CBLL1 (Cbl proto-oncogene like 1) and CBLL2 (Cbl proto-oncogene like 2). [provided by Alliance of Genome Resources, Nov 2024]
    Orthologs
    all
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    Genomic context

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    See Hakai in Genome Data Viewer
    Location:
    37F2-37F2; 2-54 cM
    Exon count:
    2
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2L NT_033779.5 (19580612..19582474, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2L NT_033779.4 (19580612..19582474, complement)

    Chromosome 2L - NT_033779.5Genomic Context describing neighboring genes Neighboring gene spitz Neighboring gene mir-2b-2 stem loop Neighboring gene msb1l Neighboring gene Phosphomevalonate kinase Neighboring gene Proliferating cell nuclear antigen 2 Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila. Kaido M, et al. Genes Cells, 2009 Sep. PMID 19682089
    2. Hakai is required for stabilization of core components of the m(6)A mRNA methylation machinery. Bawankar P, et al. Nat Commun, 2021 Jun 18. PMID 34145251, Free PMC Article
    3. Role of Hakai in m(6)A modification pathway in Drosophila. Wang Y, et al. Nat Commun, 2021 Apr 12. PMID 33846330, Free PMC Article
    4. Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m(6)A machinery component Wtap/Fl(2)d. Knuckles P, et al. Genes Dev, 2018 Mar 1. PMID 29535189, Free PMC Article
    5. Systematic assessment of transcriptomic and metabolic reprogramming by blue light exposure coupled with aging. Huang J, et al. PNAS Nexus, 2023 Dec. PMID 38059264, Free PMC Article

    See all (30) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Hakai is required for stabilization of core components of the m(6)A mRNA methylation machinery.
      Title: Hakai is required for stabilization of core components of the m(6)A mRNA methylation machinery.
    2. Role of Hakai in m(6)A modification pathway in Drosophila.
      Title: Role of Hakai in m(6)A modification pathway in Drosophila.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables cadherin binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin-protein transferase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables zinc ion binding ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in actin filament organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in chitin-based cuticle development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in dorsal closure IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in epithelial cell migration, open tracheal system IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in epithelium development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in female sex determination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in head involution IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in midgut development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein ubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein ubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein ubiquitination ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in regulation of alternative mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of cell adhesion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in segmentation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of RNA N6-methyladenosine methyltransferase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasmic vesicle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in perinuclear region of cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    hakai
    Names
    CG10263-PA
    CG10263-PC
    CG10263-PE
    CG10263-PF
    Hakai-PA
    Hakai-PC
    Hakai-PE
    Hakai-PF

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033779.5 Reference assembly

      Range
      19580612..19582474 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_176061.3 → NP_788075.2  hakai, isoform E [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_788075.2

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8INW0
      Conserved Domains (1) summary
      cd00162
      Location:170 → 207
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

    2. NM_136149.3 → NP_609993.1  hakai, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_609993.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q9VIT1
      Related
      FBpp0080804
      Conserved Domains (1) summary
      cd00162
      Location:170 → 207
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

    3. NM_001273664.1 → NP_001260593.1  hakai, isoform F [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001260593.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      M9PBE2, Q86NQ9, Q8INV9, Q8INW0, Q95RE3, Q9VIT1
      Conserved Domains (1) summary
      cd16508
      Location:178 → 216
      RING-HC_HAKAI_like; RING finger, HC subclass, found in E3 ubiquitin-protein ligase Hakai, zinc finger protein 645 (ZNF645), and similar proteins

    4. NM_165312.2 → NP_724217.1  hakai, isoform C [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_724217.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8INV9
      Related
      FBpp0080805
      Conserved Domains (1) summary
      cd00162
      Location:179 → 216
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    M9PBE2.1 M9PBE2

    Gene LinkOut

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