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    Spib Spi-B transcription factor (Spi-1/PU.1 related) [ Mus musculus (house mouse) ]

    Gene ID: 272382, updated on 28-Oct-2024

    Summary

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    Official Symbol
    Spibprovided by MGI
    Official Full Name
    Spi-B transcription factor (Spi-1/PU.1 related)provided by MGI
    Primary source
    MGI:MGI:892986
    See related
    Ensembl:ENSMUSG00000008193 AllianceGenome:MGI:892986
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Spi-B
    Summary
    Enables DNA-binding transcription factor activity. Involved in immature B cell differentiation. Acts upstream of or within macrophage differentiation and regulation of DNA-templated transcription. Predicted to be part of transcription regulator complex. Predicted to be active in nucleus. Is expressed in brain and liver. Orthologous to human SPIB (Spi-B transcription factor). [provided by Alliance of Genome Resources, Oct 2024]
    Expression
    Biased expression in spleen adult (RPKM 101.9), mammary gland adult (RPKM 21.1) and 2 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Spib in Genome Data Viewer
    Location:
    7 B3; 7 28.83 cM
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (44175419..44181511, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (44525995..44532087, complement)

    Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_18800 Neighboring gene ER membrane protein complex subunit 10 Neighboring gene golgi associated RAB2 interactor 5A Neighboring gene myosin binding protein C, fast-type Neighboring gene STARR-positive B cell enhancer ABC_E10437 Neighboring gene polymerase (DNA directed), delta 1, catalytic subunit Neighboring gene microRNA 7053 Neighboring gene STARR-positive B cell enhancer ABC_E1339 Neighboring gene STARR-positive B cell enhancer ABC_E8170 Neighboring gene STARR-positive B cell enhancer ABC_E2234 Neighboring gene nuclear receptor subfamily 1, group H, member 2

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Inhibition of microfold cells ameliorates early pathological phenotypes by modulating microglial functions in Alzheimer's disease mouse model. Kim N, et al. J Neuroinflammation, 2023 Nov 27. PMID 38012646, Free PMC Article
    2. Spi-B is critical for plasmacytoid dendritic cell function and development. Sasaki I, et al. Blood, 2012 Dec 6. PMID 23065153
    3. Transcription factor Spi-B-dependent and -independent pathways for the development of Peyer's patch M cells. Sato S, et al. Mucosal Immunol, 2013 Jul. PMID 23212199
    4. Limitation of immune tolerance-inducing thymic epithelial cell development by Spi-B-mediated negative feedback regulation. Akiyama N, et al. J Exp Med, 2014 Nov 17. PMID 25385757, Free PMC Article
    5. Enforced expression of Spi-B reverses T lineage commitment and blocks beta-selection. Lefebvre JM, et al. J Immunol, 2005 May 15. PMID 15879115

    See all (65) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. SpiB regulates the expression of B-cell-related genes and increases the longevity of memory B cells.
      Title: SpiB regulates the expression of B-cell-related genes and increases the longevity of memory B cells.
    2. Opposing Roles for the Related ETS-Family Transcription Factors Spi-B and Spi-C in Regulating B Cell Differentiation and Function.
      Title: Opposing Roles for the Related ETS-Family Transcription Factors Spi-B and Spi-C in Regulating B Cell Differentiation and Function.
    3. The mechanism of action of Spi-B in the transcriptional activation of the interferon-alpha4 gene.
      Title: The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene.
    4. Mice lacking both PU.1 and SpiB in mature B cells do not generate germinal centers and high-affinity antibody after protein immunization. PU.1 and SpiB double-deficient B cells have a survival defect after engagement of CD40 or Toll-like receptors (TLR), despite paradoxically enhanced plasma cell differentiation.
      Title: Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB.
    5. Mechanistic analysis indicated that E47 activated expression of the transcription factor Spi-B and the suppressor of cytokine signaling 3 (SOCS3), which both downregulated Foxp3 expression. These findings demonstrate that the balance of Id3 and E47 controls the maintenance of Foxp3 expression in Treg cells and, thus, contributes to Treg cell plasticity.
      Title: Id3 Maintains Foxp3 Expression in Regulatory T Cells by Controlling a Transcriptional Network of E47, Spi-B, and SOCS3.
    6. Our results suggest that complementary biological functions of PU.1 and Spi-B may be explained by their interaction with a similar set of regions in the genome of B cells.
      Title: Genome-wide comparison of PU.1 and Spi-B binding sites in a mouse B lymphoma cell line.
    7. Nfkb1 transcriptional activation by PU.1 and Spi-B promotes toll-like receptor-mediated B cell proliferation.
      Title: Nfkb1 activation by the E26 transformation-specific transcription factors PU.1 and Spi-B promotes Toll-like receptor-mediated splenic B cell proliferation.
    8. Data show that transcription factor Spi-B-mediated negative feedback regulation limited medullary thymic epithelial cells (mTECs) development.
      Title: Limitation of immune tolerance-inducing thymic epithelial cell development by Spi-B-mediated negative feedback regulation.
    9. there is a small M-cell population with developmental regulation that is Spi-B independent; however, Spi-B is probably a candidate master regulator of M-cell functional maturation and development by another pathway.
      Title: Transcription factor Spi-B-dependent and -independent pathways for the development of Peyer's patch M cells.
    10. Data indicate that the developmental defects of plasmacytoid dendritic cells (pDC) in Spi-B-deficient mice were more prominent in the bone marrow.
      Title: Spi-B is critical for plasmacytoid dendritic cell function and development.
    Submit: New GeneRIF Correction See all GeneRIFs (20)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 
    • Targeted (4)  1 citation

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA-binding transcription activator activity, RNA polymerase II-specific ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables DNA-binding transcription factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables sequence-specific DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sequence-specific double-stranded DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in cell differentiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in immature B cell differentiation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within macrophage differentiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of transcription regulator complex IC
    Inferred by Curator
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    transcription factor Spi-B
    Names
    Ets transcription factor Spi-B
    Spi-1/PU.1 related

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_019866.1 → NP_063919.1  transcription factor Spi-B

      See identical proteins and their annotated locations for NP_063919.1

      Status: VALIDATED

      Source sequence(s)
      CAAA01070168
      Consensus CDS
      CCDS21209.1
      UniProtKB/Swiss-Prot
      O35906, O35907, O35909, O55199
      UniProtKB/TrEMBL
      B9EIW0
      Related
      ENSMUSP00000035539.5, ENSMUST00000035323.6
      Conserved Domains (1) summary
      smart00413
      Location:173 → 261
      ETS; erythroblast transformation specific domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000073.7 Reference GRCm39 C57BL/6J

      Range
      44175419..44181511 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030242628.1 → XP_030098488.1  transcription factor Spi-B isoform X7

      UniProtKB/TrEMBL
      B9EIW0
      Conserved Domains (1) summary
      smart00413
      Location:155 → 243
      ETS; erythroblast transformation specific domain

    2. XM_006540939.3 → XP_006541002.1  transcription factor Spi-B isoform X9

    3. XM_006540933.3 → XP_006540996.1  transcription factor Spi-B isoform X2

      UniProtKB/TrEMBL
      A0A0X1KG64
      Related
      ENSMUSP00000096084.4, ENSMUST00000098483.9
      Conserved Domains (1) summary
      smart00413
      Location:191 → 279
      ETS; erythroblast transformation specific domain

    4. XM_006540938.4 → XP_006541001.1  transcription factor Spi-B isoform X8

    5. XM_006540934.4 → XP_006540997.1  transcription factor Spi-B isoform X4

      UniProtKB/TrEMBL
      A0A0X1KG64
      Conserved Domains (1) summary
      smart00413
      Location:174 → 262
      ETS; erythroblast transformation specific domain

    6. XM_006540932.5 → XP_006540995.1  transcription factor Spi-B isoform X1

      UniProtKB/TrEMBL
      A0A0X1KG64
      Conserved Domains (1) summary
      smart00413
      Location:192 → 280
      ETS; erythroblast transformation specific domain

    7. XM_030242627.2 → XP_030098487.1  transcription factor Spi-B isoform X6

      UniProtKB/TrEMBL
      A0A0U1RPG0
      Conserved Domains (1) summary
      smart00413
      Location:156 → 244
      ETS; erythroblast transformation specific domain

    8. XM_006540935.5 → XP_006540998.1  transcription factor Spi-B isoform X3

      UniProtKB/TrEMBL
      A0A0U1RPG0
      Related
      ENSMUSP00000145968.2, ENSMUST00000205506.2
      Conserved Domains (1) summary
      smart00413
      Location:174 → 262
      ETS; erythroblast transformation specific domain

    9. XM_006540936.4 → XP_006540999.1  transcription factor Spi-B isoform X5

      UniProtKB/TrEMBL
      B9EIW0
      Conserved Domains (1) summary
      smart00413
      Location:164 → 252
      ETS; erythroblast transformation specific domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O35906.2 GenPept UniProtKB/Swiss-Prot:O35906

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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