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    dnaE DNA polymerase III subunit alpha [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944877, updated on 19-Sep-2024

    Summary

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    Official Symbol
    dnaE
    Official Full Name
    DNA polymerase III subunit alpha
    Primary source
    ECOCYC:EG10238
    Locus tag
    b0184
    See related
    ASAP:ABE-0000624
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0183; polC; sdgC
    Summary
    Has a PHP domin, like YcdX. [More information is available at EcoGene: EG10238]. The alpha subunit of DNA polymerase III catalyzes the polymerase activity of the holoenzyme complex . [More information is available at EcoCyc: EG10238].
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    Genomic context

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    Sequence:
    NC_000913.3 (205126..208608)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase Neighboring gene lipid A disaccharide synthase Neighboring gene RNase HII Neighboring gene acetyl-CoA carboxyltransferase subunit alpha Neighboring gene lysine decarboxylase 2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. DNA Polymerase α Subunit Residues and Interactions Required for Efficient Initiation Complex Formation Identified by a Genetic Selection. Lindow JC, et al. J Biol Chem, 2015 Jul 3. PMID 25987558, Free PMC Article
    2. A structural role for the PHP domain in E. coli DNA polymerase III. Barros T, et al. BMC Struct Biol, 2013 May 14. PMID 23672456, Free PMC Article
    3. Escherichia coli DNA polymerase III is responsible for the high level of spontaneous mutations in mutT strains. Yamada M, et al. Mol Microbiol, 2012 Dec. PMID 23043439, Free PMC Article
    4. Single-molecule studies reveal the function of a third polymerase in the replisome. Georgescu RE, et al. Nat Struct Mol Biol, 2011 Dec 11. PMID 22157955, Free PMC Article
    5. Optical tweezers experiments resolve distinct modes of DNA-protein binding. McCauley MJ, et al. Biopolymers, 2009 Apr. PMID 19173290, Free PMC Article

    See all (135) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. results support a model in which the pol and exo activities of pol III core are effectively independent, and in which recognition of the 3' end of the primer by either alpha or epsilon is governed by the primer stability.
      Title: Single-molecule mechanochemical characterization of E. coli pol III core catalytic activity.
    2. Mutation in PHP domain of Pol III alpha ablated interaction with the proofreading subunit.
      Title: DNA Polymerase α Subunit Residues and Interactions Required for Efficient Initiation Complex Formation Identified by a Genetic Selection.
    3. Data show that the tau (tau), alpha (alpha), epsilon (epsilon), and theta (theta;) subunits of DNA Polymerase III can be assembled in vivo, yielding the trimeric tau3alpha3epsilon3theta;3 complex.
      Title: Simultaneous ternary extension of DNA catalyzed by a trimeric replicase assembled in vivo.
    4. The results suggest that competition between UmuD and ssDNA for DNA polymerase III alpha binding is a new mechanism for polymerase exchange.
      Title: Polymerase manager protein UmuD directly regulates Escherichia coli DNA polymerase III α binding to ssDNA.
    5. The PHP domain is a major structural element in Pol III and its integrity modulates both the stability and activity of the polymerase.
      Title: A structural role for the PHP domain in E. coli DNA polymerase III.
    6. Authors demonstrate for the first time that the major DNA polymerases (Pol I and Pol III) and DNA ligase are directly involved with oligo recombination.
      Title: Bacterial DNA polymerases participate in oligonucleotide recombination.
    7. Trigger factor dependent refolding of bacterial luciferases in Escherichia coli cells: kinetics, efficiency and effect of the bichaperone system, DnaKJE-ClpB
      Title: [Trigger factor dependent refolding of bacterial luciferases in Escherichia coli cells: kinetics, efficiency and effect of the bichaperone system, DnaKJE-ClpB].
    8. A structural model of the alphaepsilontheta;:beta2 replicase complex reveals a compact, but still flexible, structure of the complex.
      Title: Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, epsilon, θ and β reveals a highly flexible arrangement of the proofreading domain.
    9. Authors report that DNA pol III incorporates 8-oxo-dGTP approximately 20 times more efficiently opposite template A compared with template C.
      Title: Escherichia coli DNA polymerase III is responsible for the high level of spontaneous mutations in mutT strains.
    10. Authors propose that the interaction between DNA polymerase III alpha and UmuD contributes to the transition between replicative and translesion synthesis polymerases by removing DNA polymerase III alpha from the beta clamp.
      Title: Selective disruption of the DNA polymerase III α-β complex by the umuD gene products.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    NOT enables 3'-5' exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed DNA polymerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA-templated DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in lagging strand elongation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in leading strand elongation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DNA polymerase III complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of DNA polymerase III, core complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of DNA polymerase III, core complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of replisome NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA polymerase III subunit alpha
    NP_414726.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      205126..208608
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414726.1 DNA polymerase III subunit alpha [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414726.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6M9X2B0, W1X3D1
      Conserved Domains (1) summary
      PRK05673
      Location:5 → 1151
      dnaE; DNA polymerase III subunit alpha; Validated
    Nucleotide Protein
    Heading Accession and Version

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