Nox4 NADPH oxidase 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nox4provided by RGD
Official Full Name: NADPH oxidase 4provided by RGD
Primary source: RGD:620600
See related: EnsemblRapid:ENSRNOG00000013925 AllianceGenome:RGD:620600
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables NAD(P)H oxidase H2O2-forming activity and superoxide-generating NAD(P)H oxidase activity. Involved in several processes, including cellular response to cAMP; cellular response to gamma radiation; and cellular response to mercury ion. Located in several cellular components, including endoplasmic reticulum; focal adhesion; and stress fiber. Part of NADPH oxidase complex. Used to study hypertension. Biomarker of hypertension; metabolic dysfunction-associated steatohepatitis; obesity; and steatotic liver disease. Orthologous to human NOX4 (NADPH oxidase 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Kidney (RPKM 604.2), Lung (RPKM 75.7) and 1 other tissue See more
Orthologs: human mouse all
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Genomic context

Location:: 1q32

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (150313736..150491480)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (140900886..141078844)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (150796359..150976186)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Intermittent short-duration reoxygenation relieves high-altitude pulmonary hypertension via NOX4/H2O2/PPAR-gamma axis.
Title: Intermittent short-duration reoxygenation relieves high-altitude pulmonary hypertension via NOX4/H2O2/PPAR-γ axis.
Advanced glycated end-products inhibit dilation through constitutive endothelial RAGE and Nox1/4 in rat isolated skeletal muscle arteries.
Title: Advanced glycated end-products inhibit dilation through constitutive endothelial RAGE and Nox1/4 in rat isolated skeletal muscle arteries.
Hypoxia stabilizes the H2 O2 -producing oxidase Nox4 in cardiomyocytes via suppressing autophagy-related lysosomal degradation.
Title: Hypoxia stabilizes the H(2) O(2) -producing oxidase Nox4 in cardiomyocytes via suppressing autophagy-related lysosomal degradation.
Interruption of TRPC6-NFATC1 signaling inhibits NADPH oxidase 4 and VSMCs phenotypic switch in intracranial aneurysm.
Title: Interruption of TRPC6-NFATC1 signaling inhibits NADPH oxidase 4 and VSMCs phenotypic switch in intracranial aneurysm.
Maternal exercise represses Nox4 via SIRT1 to prevent vascular oxidative stress and endothelial dysfunction in SHR offspring.
Title: Maternal exercise represses Nox4 via SIRT1 to prevent vascular oxidative stress and endothelial dysfunction in SHR offspring.
VEGF-A: A Novel Mechanistic Link Between CYP2C-Derived EETs and Nox4 in Diabetic Kidney Disease.
Title: VEGF-A: A Novel Mechanistic Link Between CYP2C-Derived EETs and Nox4 in Diabetic Kidney Disease.
Functional Significance of Angiotensin Receptor Type 2 in the Neuroplasticity of Autonomic Ganglia in (mRen2)27 Transgenic Hypertensive Rats.
Title: Functional Significance of Angiotensin Receptor Type 2 in the Neuroplasticity of Autonomic Ganglia in (mRen2)27 Transgenic Hypertensive Rats.
Advanced oxidation protein products induce annulus fibrosus cell senescence through a NOX4-dependent, MAPK-mediated pathway and accelerate intervertebral disc degeneration.
Title: Advanced oxidation protein products induce annulus fibrosus cell senescence through a NOX4-dependent, MAPK-mediated pathway and accelerate intervertebral disc degeneration.
MicroRNA-454 modulates the oxidative stress and neuronal apoptosis after cerebral ischemia/reperfusion injury via targeting NADPH oxidase 4 (NOX4).
Title: MicroRNA-454 modulates the oxidative stress and neuronal apoptosis after cerebral ischemia/reperfusion injury via targeting NADPH oxidase 4 (NOX4).
Role of Nox4 in High Calcium-Induced Renal Oxidative Stress Damage and Crystal Deposition.
Title: Role of Nox4 in High Calcium-Induced Renal Oxidative Stress Damage and Crystal Deposition.

Submit: New GeneRIF Correction See all GeneRIFs (119)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU047304 (e-PCR)
- UniSTS:110640

Nox4 (e-PCR), detects polymorphism
- UniSTS:525524

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables NAD(P)H oxidase H2O2-forming activity	IDA Inferred from Direct Assay more info	PubMed
enables NAD(P)H oxidase H2O2-forming activity	ISO Inferred from Sequence Orthology more info
enables NADPH oxidase H202-forming activity	IEA Inferred from Electronic Annotation more info
enables heme binding	ISO Inferred from Sequence Orthology more info
enables heme binding	ISS Inferred from Sequence or Structural Similarity more info
enables modified amino acid binding	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine kinase binding	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase binding	ISO Inferred from Sequence Orthology more info
enables superoxide-generating NAD(P)H oxidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables superoxide-generating NAD(P)H oxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables superoxide-generating NAD(P)H oxidase activity	IEA Inferred from Electronic Annotation more info
NOT enables superoxide-generating NAD(P)H oxidase activity	ISO Inferred from Sequence Orthology more info
enables superoxide-generating NAD(P)H oxidase activity	ISO Inferred from Sequence Orthology more info
enables superoxide-generating NADPH oxidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in bone resorption	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within bone resorption	ISO Inferred from Sequence Orthology more info
involved_in cardiac muscle cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cardiac muscle cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in cell morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in cell morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in cell morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to cAMP	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to gamma radiation	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to glucose stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to interleukin-1	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to mercury ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to transforming growth factor beta stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in defense response	IBA Inferred from Biological aspect of Ancestor more info
involved_in gene expression	ISO Inferred from Sequence Orthology more info
involved_in heart process	IEA Inferred from Electronic Annotation more info
involved_in heart process	ISO Inferred from Sequence Orthology more info
involved_in homocysteine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of DNA biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of DNA biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ERK1 and ERK2 cascade	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of MAP kinase activity	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of MAP kinase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of hydrogen peroxide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein tyrosine kinase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of smooth muscle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of stress fiber assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reactive oxygen species metabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in superoxide anion generation	IBA Inferred from Biological aspect of Ancestor more info
involved_in superoxide anion generation	IEA Inferred from Electronic Annotation more info
involved_in superoxide anion generation	ISO Inferred from Sequence Orthology more info
involved_in superoxide anion generation	ISS Inferred from Sequence or Structural Similarity more info
involved_in superoxide metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in superoxide metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in superoxide metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of NADPH oxidase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of NADPH oxidase complex	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cell periphery	IEA Inferred from Electronic Annotation more info
located_in cell periphery	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in sarcoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in stress fiber	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: NADPH oxidase 4

Names: kidney oxidase-1; kidney superoxide-producing NADPH oxidase; kox-1

NP_445976.1

EC 1.6.3.1

XP_008757865.1

EC 1.6.3.1

XP_038948871.1

EC 1.6.3.1

XP_038948872.1

EC 1.6.3.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053524.1 → NP_445976.1 NADPH oxidase 4

See identical proteins and their annotated locations for NP_445976.1

Status: PROVISIONAL

Source sequence(s)

AY027527

UniProtKB/Swiss-Prot

Q924V1, Q99M78

UniProtKB/TrEMBL

A0A8I5ZPH8, A6I5Y4

Related

ENSRNOP00000085650.1, ENSRNOT00000102096.2

Conserved Domains (2) summary

cd06186
Location:311 → 577

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

pfam01794
Location:69 → 204

Ferric_reduct; Ferric reductase like transmembrane component

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

150313736..150491480

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008759643.4 → XP_008757865.1 NADPH oxidase 4 isoform X3

UniProtKB/TrEMBL

A6I5Y3

Conserved Domains (2) summary

cd06186
Location:311 → 622

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

pfam01794
Location:69 → 204

Ferric_reduct; Ferric reductase like transmembrane component
XM_039092943.2 → XP_038948871.1 NADPH oxidase 4 isoform X1

UniProtKB/TrEMBL

A0A8I5ZPH8

Related

ENSRNOP00000106633.1, ENSRNOT00000131019.1

Conserved Domains (2) summary

cd06186
Location:311 → 556

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

pfam01794
Location:69 → 204

Ferric_reduct; Ferric reductase like transmembrane component
XM_039092944.2 → XP_038948872.1 NADPH oxidase 4 isoform X2

UniProtKB/TrEMBL

A0A8I5ZPH8

Conserved Domains (2) summary

cd06186
Location:237 → 503

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

pfam01794
Location:17 → 130

Ferric_reduct; Ferric reductase like transmembrane component